MARC details
| 000 -LEADER |
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| fixed length control field |
04805ntm a2200337 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251125111024.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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t||||fr|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250203t20242024my a|||fr|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0009998 (Local) |
| Qualifying information |
Hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMPSA |
| Language of cataloging |
eng |
| Transcribing agency |
UMPSA |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FTKKP .S89 2024 r Thesis |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Wan Nur Suzilla Bt Wan Yusuf, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
The antiproliferative study of quercus infectoria galls crude and micelle extract hydrogel beads against selected breast cancer cells / |
| Statement of responsibility, etc. |
Wan Nur Suzilla Bt Wan Yusuf |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Kuantan Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMPSA, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2024 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Date of production, publication, distribution, manufacture, or copyright notice |
© 2024 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xv, 189 pages : |
| Other physical details |
illustration ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| Source |
rda |
| File type |
text file |
| Encoding format |
PDF |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Chemical and Process Engineering Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Doctor of Philosophy) -- Universiti Malaysia Pahang – 2024 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Hydrogel beads are used in scientific studies to investigate the potential of natural extracts in inhibiting cancer cell growth. They provide stability and transportation by enclosing the extract within a network, ensuring its antiproliferative properties. They can also be engineered for controlled release, ensuring consistent antiproliferative effects. Additionally, they prevent agglutination, ensuring uniform distribution of the extract, and potentially enhancing its efficacy. Hence, in this study, Quercus infectoria (QI) galls hydrogel beads incorporated with QI galls crude and micelle extracts were developed to overcome the micelle aggregation and stability problem as well as a new alternative in cancer treatment. QI galls crude extract was produced using maceration extraction with 70% aqueous ethanol while QI galls crude extract loaded micelle was formulated using solvent casting method and then both extracts were characterized for their physicochemical properties. Then, the QI galls crude and micelle extract were incorporated into hydrogel beads via crosslinking action between polymer (pectin) and crosslinker (zinc acetate). All crude extract, micelles and hydrogel beads were tested against selected breast cancer cells (MCF-7 and MDA-MB-231) to determine the antiproliferative activity using MTT Assay as well as for apoptosis activity via FITCAnnexin V staining. From the findings, it was observed that the QI galls crude extract, micelles and hydrogel beads exhibited good antiproliferative activity against breast cancer cells (MCF-7 and MDA-MB-231) but showed no cytotoxicity against normal cell line, L929 apart from induced the cell death mechanism through apoptosis. All formulated micelles showed a good size range (below 20 nm), with polydispersity index (PDI) value between 0.1-0.4 indicating narrow distribution and negatively charged. Based on the surface chemistry analysis, it was observed that all formulated micelles, had almost similar relative ratio amongst the suggested chemical bonds and in accordance with those reported in pure vitamin E TPGS. For extract loading, it was showed that Quercus infectoria Vitamin E TPGS (QIT) micelle gave highest loading (110.41 µg/ml) as compared to other micelles and the surface morphology by Transmission Electron Microscope (TEM), showed all formulated micelles were in moderate uniformity with nearly spherical shape. Controlled drug release profile showed all micelles demonstrated biphasic release pattern while the cellular uptake was increased with prolonged incubation time. QIT micelle showed highest cytotoxic activity (IC50 8.28±0.82 µg/ml) against MCF-7 cells with faster onset of action as compared to MDA-MB-231 (IC50 11.09±0.73 µg/ml) and induced apoptotic cell death mechanism with no toxicity effect against normal cells, L929. For the hydrogel beads formulation, two different hydrogel beads were formulated, using QI crude (QIC) and micelle (QIM) extract. From the findings, it was showed both formulated beads, demonstrated good yield, morphology, swelling ability, swelling time and moisture content (below 20%). As conclusion, a novel polymeric hydrogel beads incorporated with QI galls crude and micelle extract with good antiproliferative activity against selected breast cancer cells were successfully formulated and presents a promising strategy for safeguarding the integrity of extracts by improving their stability and effectively preventing undesirable aggregation. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Chemical and Process Engineering Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Theses |
| General subdivision |
Dissertations |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |