MARC details
| 000 -LEADER |
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03687nam a2200349 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20260120115342.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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t||||fs|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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260120t20252025my a|||fs|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0010286 (Local) |
| Qualifying information |
Hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMPSA |
| Language of cataloging |
eng |
| Transcribing agency |
UMPSA |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .A456 2025 r Bc. |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Amin Nur Husna Binti Airol Azamir, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
In silico characterization of phenylacetaldehyde synthases of selected orchids / |
| Statement of responsibility, etc. |
Amin Nur Husna Binti Airol Azamir |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Kuantan, Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMPSA, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2025 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Date of production, publication, distribution, manufacture, or copyright notice |
© 2025 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiv, 95 pages : |
| Other physical details |
illustrations ; |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| Source |
rda |
| File type |
text file |
| Encoding format |
PDF |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Sciences and Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Include bibliographical reference |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Phenylacetaldehyde synthase (PAAS) is an enzyme essential for producing phenylacetaldehyde, a key component of floral scent. It acts as a bifunctional enzyme, efficiently coupling phenylalanine decarboxylation to oxidation. This process yields phenylacetaldehyde, CO2, ammonia, and hydrogen peroxide in precise stoichiometric amounts. Despite its importance, the presence and characteristics of PAAS in various flowers remain underexplored. This study investigated the phenylacetaldehyde synthase (PAAS) and its homologous proteins in five orchid species: Vanda Mimi Palmer, Phalaenopsis equestris, Dendrobium nobile, Dendrobium candidum, and Iris pallida, using an in silico approach. Amino acid sequences available in the NCBI GenBank were utilized to perform homology modeling and predict PAAS's three-dimensional (3D) structures and related proteins. The reliability of these structural models was validated using VERIFY 3D, PROCHECK, and ERRAT tools. This study aims to predict the 3Dprotein structure of selected orchids’ phenylacetaldehyde synthase and homologous proteins using a homology modeling approach and to investigate the binding positions of potential ligands to the 3D structure of the selected orchids’ phenylacetaldehyde synthases by molecular docking. Active pocket regions (R1 and R2) were identified using CASTp, whereas R1 is the predicted pocket binding site of the selected orchid phenylacetaldehyde synthase and homologous proteins while R2 is the predicted pocket binding site of the 6eem, and molecular docking experiments were conducted to evaluate the binding sites and affinities of potential substrates (phenylalanine, tryptophan, and tyrosine) and their products (phenylacetaldehyde, tryptamine, and tyramine). Finally, protein-ligand docking studies were conducted with Autodock Vina to predict the orientation of a ligand bound to the protein and compare it with the predicted active site pocket analysis. This study contributes to a deeper understanding of PAAS's molecular and functional properties and its homologous proteins in orchids. These insights enhance knowledge of floral scent biosynthesis, particularly the enzymatic pathways involving phenylacetaldehyde synthase, and provide valuable information for potential applications in horticulture, the fragrance industry, and related fields |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Sciences and Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Final Year Report |
| General subdivision |
Dissertations |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
http://umpir.ump.edu.my/id/eprint/45596 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Final Year Report |