MARC details
| 000 -LEADER |
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| fixed length control field |
03374nam a22003497a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20260126162736.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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| fixed length control field |
t||||fs|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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| fixed length control field |
ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
260126t20252025my a|||fs|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0010319 (Local) |
| Qualifying information |
Hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMPSA |
| Language of cataloging |
eng |
| Transcribing agency |
UMPSA |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .A357 2025 r Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nurul Afiqah Binti Azfi, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
In silico characterizationof selected orchids’ sesquiterpene synthases / |
| Statement of responsibility, etc. |
Nurul Afiqah Binti Azfi |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Kuantan, Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMPSA, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2025 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
© 2025 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xv, 96 pages : |
| Other physical details |
illustrations ; |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
|---|
| Source |
rda |
| File type |
text file |
| Encoding format |
PDF |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Sciences and Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Include bibliographical reference |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The structural and functional characterization of sesquiterpene synthases (STSs), which are essential enzymes in the biosynthesis of terpenoid compounds, is still lacking, especially in orchids. Using computational techniques like as molecular docking and homology modeling, this study fills the information gap by forecasting the ligand-binding processes and three-dimensional structures of six representative orchid STSs. The Vanda Mimi Palmer STS achieved 100% sequence identity in BLASTp analysis, and other important proteins examined include sesquiterpene synthases from Vanda Mimi Palmer, Vanda Small Boy Leong, and Phalaenopsis equestris. Phylogenetic studies have shown that orchid STSs are evolutionarily maintained and distinct from their non-orchid counterparts due to their diversification within the Orchidaceae family. SWISS-MODEL, Phyre, and BLAST-PDB were used for homology modeling, which determined that 7XKW was the best template because of its high sequence identity (up to 45%) and structural significance. ERRAT, PROCHECK, and VERIFY 3D quality evaluation verified SWISS-MODEL's excellence in producing dependable, stereochemically accurate models. At conserved active regions (R1 and R2), molecular docking experiments demonstrated substantial binding affinities for important ligands including farnesyl pyrophosphate (S1) and germacrene D (P1). Other ligands, such as linalool and ocimene, showed lesser affinities, whereas these ligands showed consistent binding patterns and interactions that are essential for sesquiterpene production. The results offer a thorough comprehension of the structural characteristics and enzymatic processes of orchid STSs. This study lays the groundwork for future experimental verification, supporting the investigation of STS functionality and biotechnology applications, specifically in terpenoid synthesis and scent generation. The work highlights how in silico methods may be used to advance structural biology and uncover the secondary metabolites found in plants' biotechnological potential |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Sciences and Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Final Year Report |
| General subdivision |
Dissertations |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
http://umpir.ump.edu.my/id/eprint/45697 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Final Year Report |