Analysis of bacterial community composition in fermented mustard green through amplicon sequencing / (Record no. 104245)

MARC details
000 -LEADER
fixed length control field 03573ntm a2200325 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field MY-KuUP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260227111402.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field t||||fs|||| 000 0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 260226t20252025my a|||fs|||| 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0010393 (Local)
Qualifying information Hardback
040 ## - CATALOGING SOURCE
Original cataloging agency UMPSA
Language of cataloging eng
Transcribing agency UMPSA
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FIST .S46 2025 r Bc.
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Sen Yung Jie,
Relator term author.
245 10 - TITLE STATEMENT
Title Analysis of bacterial community composition in fermented mustard green through amplicon sequencing /
Statement of responsibility, etc. Sen Yung Jie
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Kuantan, Pahang :
Name of producer, publisher, distributor, manufacturer UMPSA,
Date of production, publication, distribution, manufacture, or copyright notice 2025
264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice © 2025
300 ## - PHYSICAL DESCRIPTION
Extent xi, 72 pages :
Other physical details illustrations ;
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
347 ## - DIGITAL FILE CHARACTERISTICS
Source rda
File type text file
Encoding format PDF
500 ## - GENERAL NOTE
General note Faculty of Industrial Sciences and Technology
502 ## - DISSERTATION NOTE
Dissertation note Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Include bibliographical reference
520 3# - SUMMARY, ETC.
Summary, etc. This study provides a comprehensive examination of fermented mustard greens by simultaneously analyzing their liquid and solid fractions to elucidate microbial diversity, community composition, and functional attributes. DNA was extracted via the QIAamp PowerFecal Pro Kit, amplified with 16S rRNA primers targeting the V3 region, and subjected to high-throughput sequencing and QIIME2-based taxonomic classification. Alpha-diversity metrics (e.g., Chao1, ACE) revealed only modest, statistically non-significant differences between the brine and the leafy fractions, indicating that both harbors comparably rich microbial communities. Beta-diversity analyses (Bray–Curtis PCoA) showed moderate compositional distinctions (ANOSIM R ≈ 0.55, p < 0.1), suggesting that with a larger sample size, these differences might become more pronounced. Taxonomic profiling underscored the dominance of beneficial bacterial phyla—most notably Actinobacteriota, Proteobacteria, and Firmicutes—reinforced by multiple probiotic genera such as Bifidobacterium, Weissella, Ligilactobacillus, Faecalibacterium, and Brevibacterium. Although low levels of potentially pathogenic species (e.g., Cutibacterium acnes, Streptococcus dysgalactiae, Clostridium perfringens) were detected, their relative abundances were negligible, posing minimal health risk. Functional analyses, validated by statistical significance (p < 0.05) and robust effect sizes, pinpointed key metabolic pathways—including L-Methionine Biosynthesis III, S-Adenosyl-LMethionine Biosynthesis, Pantothenate and Coenzyme A Biosynthesis, Taxadiene Biosynthesis, and Glucose/Xylose Degradation—that collectively bolster antioxidant defense, energy metabolism, and microbial stability. Enzymatic profiling further illuminated crucial enzymes (e.g., fructose-bisphosphatase, ribokinase, shikimate dehydrogenase) that aid in carbohydrate breakdown, foster beneficial microbial proliferation, and generate short-chain fatty acids vital to gut health. Overall, the findings confirm that both fractions of fermented mustard greens contribute to a resilient and functionally diverse microbiome, underscoring the potential of this food product as a probiotic-rich, metabolically active, and health-promoting option for personalized nutrition and improved gut health management
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Industrial Sciences and Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Final Year Report
General subdivision Dissertations
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Final Year Report
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Use restrictions Not for loan Permanent Location Current Location Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
  Not lost Library of Congress Classification   Restricted access   UMPLIB GAMBANG UMPLIB GAMBANG 26/02/2026   FIST .S46 2025 r Bc. T000003709 26/02/2026 26/02/2026 Restricted Collection

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