In-situ immobilised laccase on bacterial cellulose for juice phenolic compounds oxidation / (Record no. 105144)

MARC details
000 -LEADER
fixed length control field 03349ntm a2200337 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field MY-KuUP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260708180016.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field t|||||r|||| 000 0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field ta
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 260708t20252025my a|||fr|||| 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0010681 (Local)
Qualifying information Hardback
040 ## - CATALOGING SOURCE
Original cataloging agency UMPSA
Language of cataloging eng
Transcribing agency UMPSA
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FTKKP .T94 2025 r Bc.
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Hiew, Tzechingi, ,
Relator term author.
245 10 - TITLE STATEMENT
Title In-situ immobilised laccase on bacterial cellulose for juice phenolic compounds oxidation /
Statement of responsibility, etc. Hiew Tzeching
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Kuantan, Pahang :
Name of producer, publisher, distributor, manufacturer UMPSA,
Date of production, publication, distribution, manufacture, or copyright notice 2025
264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice © 2025
300 ## - PHYSICAL DESCRIPTION
Extent xiii, 60 pages :
Other physical details illustrations ;
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
347 ## - DIGITAL FILE CHARACTERISTICS
Source rda
File type text file
Encoding format PDF
500 ## - GENERAL NOTE
General note Faculty of Chemical and Process Engineering Technology
502 ## - DISSERTATION NOTE
Dissertation note Final Year Report (Bachelor of Chemical Engineering ) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Include bibliographical reference
520 3# - SUMMARY, ETC.
Summary, etc. Laccase is a multi-copper biocatalyst that catalysed the oxidation of phenolic components through a radical-mediated process. Its potential applications in various industrial sectors, including juice clarification, have been progressively developed. The naturally formed polyphenols in fruits offer nutritional benefits, but excessive presence can cause unpleasant turbidity and haze formation. This study focused on the oxidation of phenols in apple juice by utilising in-situ immobilised laccase-bacterial cellulose (BC) biocatalyst. This research aims to evaluate the effects of enzyme loading and reaction duration on the efficiency of phenolic oxidation and then investigate the reusability of the biocatalyst for juice clarification. Methodologically, laccase was immobilised on BC produced from pineapple peel waste through an in-situ synthesis process. The impact of enzyme loading was assessed by varying the biocatalyst amount and measuring the removal of phenolic content, juice clarification and colour reduction. Subsequently, the juice treatment was followed by altering different reaction times based on the best enzyme loading with the same evaluation. Then, reusability was analysed by subjecting the immobilised laccase to multiple cycles of juice treatment and monitoring its residual activity. The results indicated that increased enzyme loading improved catalytic oxidation, enhancing phenolic content removal at 40.3 ± 0.4% while reducing juice clarification for 15.9 ± 2.2% and colour for 23.3 ± 0.7%, respectively, at the best enzyme loading of 0.20 U. Additionally, 120 minutes was the best reaction duration for juice treatment by considering catalytic efficiency and economic feasibility. The result showed the deduction of 35.4 ± 3.5% of total phenolic content, 23.7 ± 1.2% of juice clarity and 26.9 ± 0.5% of its colour. The immobilised enzyme also exhibited excellent operational stability by retaining its activity up to 50% after three batch reactions. This study highlighted the utilisation of low-cost support for laccase immobilisation with application in apple juice clarification.
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Chemical and Process Engineering Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Theses
General subdivision Dissertations
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Final Year Report
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
  Not lost Library of Congress Classification     UMPLIB GAMBANG UMPLIB GAMBANG 08/07/2026   FTKKP .T94 2025 r Bc. T000004276 08/07/2026 08/07/2026 Restricted Collection

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