In-situ immobilized laccase on bacteria cellulose: varying the laccase concentration for isotherm and kinetic models determination / (Record no. 105189)

MARC details
000 -LEADER
fixed length control field 03545ntm a2200349 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field MY-KuUP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260713160342.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field t|||||r|||| 000 0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field ta
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 260713t20252025my a|||fr|||| 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0010721 (Local)
Qualifying information Hardback
040 ## - CATALOGING SOURCE
Original cataloging agency UMPSA
Language of cataloging eng
Transcribing agency UMPSA
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FTKKP .T45 2025 r Bc.
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Thivvinesh A/L Raja, , ,
Relator term author.
245 10 - TITLE STATEMENT
Title In-situ immobilized laccase on bacteria cellulose: varying the laccase concentration for isotherm and kinetic models determination /
Statement of responsibility, etc. Thivvinesh A/L Raja
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Kuantan, Pahang :
Name of producer, publisher, distributor, manufacturer UMPSA,
Date of production, publication, distribution, manufacture, or copyright notice 2025
264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice Β© 2025
300 ## - PHYSICAL DESCRIPTION
Extent 59 pages :
Other physical details illustrations ;
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
347 ## - DIGITAL FILE CHARACTERISTICS
Source rda
File type text file
Encoding format PDF
500 ## - GENERAL NOTE
General note Faculty of Chemical and Process Engineering Technology
502 ## - DISSERTATION NOTE
Dissertation note Final Year Report (Bachelor of Chemical Engineering ) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Include bibliographical reference
520 3# - SUMMARY, ETC.
Summary, etc. Enhanced enzyme activity and the potential of in-situ immobilization to reduce time and cost have motivated this study, alongside the need to deepen understanding of kinetic and isotherm models. This work aims to determine the interaction of in-situ immobilized laccase on bacterial cellulose using the best-fit isotherm model by varying enzyme concentration and to elucidate the immobilization mechanism of in-situ immobilized laccase on bacterial cellulose using the suitable-fit kinetic model by varying immobilization time. Bacteria cellulose was prepared through the fermentation of Gluconacetobacter xylinus in nutrient-rich media under static conditions. For in-situ immobilization, laccase (50-800 U/ml) was added to sterilized pineapple waste media during bacteria cellulose fermentation, and immobilization was performed over periods (0 -14 days). Experimental data obtained used to calculate for bound laccase. The bound laccase data was used to calculate π‘žπ‘’ and 𝐢𝑒 that were fitted to isotherm models, including Langmuir, Freundlich, Temkin, Dubinin-Radushkevich, Sips, Redlich-Peterson, and Hill, to evaluate the interactions governing immobilization. The Langmuir model emerged as the best fit, supported by the Sips model, which exhibited Langmuir-like behavior, indicating monolayer adsorption and the parameters values obtained for maximum adsorption capacity was 0.002 and bonding energy was ranged 0.728 and 1.042. The bound laccase obtained from experimental data was used to fit in to the kinetic models. In Kinetic studies using pseudo-first-order, pseudo-second-order, and intra-particle diffusion models revealed that the pseudo-first-order model described the physical adsorption of laccase onto bacterial cellulose, characterized by weak bonding. Additionally, the intra-particle diffusion model was validated with a high regression coefficient (0.96), identifying intra-particle diffusion as the rate-limiting step. The maximum adsorption capacity and equilibrium laccase adsorbed showed consistency between experimental and calculated values. These results confirm that the in-situ immobilization of laccase onto bacterial cellulose involves monolayer, physical adsorption with diffusion-limited kinetics.
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Chemical and Process Engineering Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Theses
General subdivision Dissertations
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Final Year Report
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
  Not lost Library of Congress Classification     UMPLIB GAMBANG UMPLIB GAMBANG 13/07/2026   FTKKP .T45 2025 r Bc. T000004314 13/07/2026 13/07/2026 Final Year Report

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