A biorefinery approach to cultivating diatom thalassiosira Pseudonana on palm oil mill effluent-based media for multiproduct recovery / (Record no. 105683)

MARC details
000 -LEADER
fixed length control field 04352ntm a2200337 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field MY-KuUP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20261007101538.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field t||||fr|||| 000 0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field ta
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 261007t20262026my a|||fr|||| 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0010822 (Local)
Qualifying information Hardback
040 ## - CATALOGING SOURCE
Original cataloging agency UMPSA
Language of cataloging eng
Transcribing agency UMPSA
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FSTI .K37 2026 r Thesis
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Karthick Murugan Palanisamy,
Relator term author.
245 12 - TITLE STATEMENT
Title A biorefinery approach to cultivating diatom thalassiosira Pseudonana on palm oil mill effluent-based media for multiproduct recovery /
Statement of responsibility, etc. Karthick Murugan Palanisamy
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Kuantan, Pahang :
Name of producer, publisher, distributor, manufacturer UMPSA,
Date of production, publication, distribution, manufacture, or copyright notice 2026
264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice © 2026
300 ## - PHYSICAL DESCRIPTION
Extent xviii, 251 pages :
Other physical details illustrations ;
Dimensions 30 cm. +
Accompanying material 1 CD-ROM.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
347 ## - DIGITAL FILE CHARACTERISTICS
Source rda
File type text file
Encoding format PDF
500 ## - GENERAL NOTE
General note Faculty of Industrial Sciences and Technology
502 ## - DISSERTATION NOTE
Dissertation note Thesis (Doctor of Philosophy) -- Universiti Malaysia Pahang - 2026
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Include bibliographical reference
520 3# - SUMMARY, ETC.
Summary, etc. Microalgal biorefineries integrated with wastewater treatment provide a sustainable method for producing valuable products and cleaning up the environment. Conversely, traditional singleproduct extraction from diatom biomass destroys biomass and underutilizes important components. In this study, the indigenous marine diatom Thalassiosira pseudonana was isolated from Kuantan coastal waters and evaluated for biomass production using palm oil mill effluent (POME)-based media and following multi-product recovery from biomass by the cascade extraction method. Key cultivation parameters were optimized to improve productivity. The strain demonstrated strong adaptability to POME-based media, and the combination of 20% POME supplemented with F/2 medium produced the highest dry biomass production (0.74±0.07 g/L) among the investigated media formulations through response surface methodology. Growth profile analysis confirmed improved specific growth rate (0.317±0.0158 μ/day), biomass productivity (0.047±0.004 g/L/d), and doubling time (3.221±1.46), indicating efficient metabolic performance in effluent-based media. A sequential cascade extraction approach was developed to maximize biomass valorisation through stepwise recovery of pigments (0.14±0.015 g/g), lipids (30%), and biosilica frustules (13%). The optimized pigment extraction process achieved a recovery yield of up to 14%, with HPLC analysis confirming the presence of chlorophyll-a (747.85 μg/ml), chlorophyll-c (143.97 μg/ml), and fucoxanthin (284.94μg/ml). The post-pigment extracted biomass was further processed for lipid recovery. Solvent optimization (30.9±3.5%) and ultrasonication (30.3±1.5%) significantly enhanced cell disruption efficiency, improving lipid recovery. GC-MS confirmed the presence of key fatty acids, including palmitic acid (215.52±10.9 μg/μL), eicosapentaenoic acid (62.45±1.65 μg/μL), and docosahexaenoic acid (10.67±2.16 μg/μL). Whereas LC-QTOF quantifies the predominant C20:0 (10.61μg/mg), palmitoleic acid (12.25 μg/mg), and eicosapentaenoic acid (5.99μg/mg). Following lipid extraction, the residual biomass was processed for biosilica recovery to ensure the silica composition and a few organic residues. FTIR revealed a strong Si-O stretch was observed at a wavelength of 1089.04 cm-1 with a strong and sharp peak because silica is the dominant component in the cleaned frustules. XRD confirms the purity of silica in frustule by high intensity with sharp peaks found at 2θ ≈ 26.63°. The FESEM EDX confirms the presence of silicon (45.50%) and oxygen (34.67%), and the primary elements. Thus, cascade biorefineries increase recovery efficiency over traditional techniques by allowing full biomass usage and minimizing waste production. Overall, the cascade biorefinery approach enhanced recovery efficiency by maximizing biomass utilization and minimizing waste. Additionally, cultivation in POME-based media enabled simultaneous biomass production and wastewater nutrient removal, supporting environmental sustainability and circular bioeconomy development.
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Industrial Sciences and Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Theses
General subdivision Dissertations
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Thesis
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Permanent Location Current Location Date acquired Total Checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not lost Library of Congress Classification   In Transit Reference UMPLIB GAMBANG UMPLIB GAMBANG 07/10/2026   FSTI .K37 2026 r Thesis T000005692 07/10/2026 1 07/10/2026 Thesis
  Not lost Library of Congress Classification   In Transit Reference UMPLIB GAMBANG UMPLIB GAMBANG 07/10/2026   CD14085 T000005693 07/10/2026 1 07/10/2026 Accompanying Material

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