MARC details
| 000 -LEADER |
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| fixed length control field |
02446nam a2200241 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000045140 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204422.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
100219t2009 my ao f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002290(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905241223 |
| Level of effort used to assign nonsubject heading access points |
shamsul |
| Level of effort used to assign subject headings |
201108030917 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201107132307 |
| Level of effort used to assign classification |
VLOAD |
| -- |
201002191023 |
| -- |
kam |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QR186.85 .S53 2009 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mohd Shamsul Husin |
| 245 10 - TITLE STATEMENT |
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| Title |
Simulation study of monoclonal antibody production using superpro, upstream process / |
| Statement of responsibility, etc. |
Mohd Shamsul Bin Husin |
| 246 3# - VARYING FORM OF TITLE |
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| Title proper/short title |
Simulation study of monoclonal antibody production using superpro, upstream process |
| Medium |
[computer file] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan : UMP, 2009 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
30 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 computer disc |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2009 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The purpose of this study is to approximate hybridoma growth kinetic model by comparing simulation result from SuperPro Designer® and experimental result. Modeling of hybridoma include calculation of mass for 1 cell and its density. Two kinetic models tested in this study; experimental correlation and de Tremblay et al. (1992). Simplification need to be made as this will allow selected model to be used in SPD. Value of μmax, KsGLN, KsGLC are 1.09 d-1 (0.05 h-1), 0.3 mM (43.85 mg/L) and 0.1 mM (18.02 mg/L) respectively for de Tremblay and for experimental correlation, their values are 0.158 h-1, 0.0016 mM (0.23 mg/L) and 12.05 mM (2170.93 mg/L) respectively. Experimental data shows no stationary phase but simulation results show stationary phase. From simulation, cell count of 195907.7 while final concentration of glucose, glutamine, ammonia and lactate are 16.38, 4.07, 3.01 and 5.59 mmol/L respectively (experimental correlation) and cell count of 196185.73 while final concentration of glucose, glutamine, ammonia and lactate are 10.73, 2.09, 6.07 and 11.30 mmol/L respectively (de Tremblay). Serious deviations occurred because of simplification on de Tremblay model and inaccurate prediction of glutamine effect on hybridoma’s growth. Metabolic reaction used without taking hybridoma real behavior into account. Simulation shows exponential phase without having going through lag phase. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Monoclonal antibodies |