MARC details
| 000 -LEADER |
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| fixed length control field |
02710nam a2200241 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000045144 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204422.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
100219t2009 my ao f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002256(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905241125 |
| Level of effort used to assign nonsubject heading access points |
shamsul |
| Level of effort used to assign subject headings |
201111241021 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201108030926 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201107132307 |
| Level of effort used to assign classification |
VLOAD |
| -- |
201002191125 |
| -- |
kam |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QP609.C39 N43 2009 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nor Fadzillah Hanis Abu Sulaiman |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of inoculum size & agitation rate on expression of recombinant chitinase in Escherichia coli / |
| Statement of responsibility, etc. |
Nor Fadzillah Hanis Binti Abu Sulaiman |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2009 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
45 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 computer disc |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2009 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Chitinase generally referred as endochitinases is randomly hydrolyzing chitin to produce N-acetyl-glucosamine. In addition, chitinase play an important role in chitin metabolism of microorganism for production of carbon and energy source. Chitinase can be found in bacteria, fungi, virus and higher plant. There are a lot of applications of chitinase either in pharmaceutical, biopestisides or food industry. For this research, chitinase was expressed in recombinant bacteria using Escherichia Coli as a host. The optimization was carried out by using response surface methodology. Focusing on two parameters which are inoculum size and agitation rate, the optimum condition was identified to enhance the process of chitinase production. The chitinase was expressed in different inoculum size at 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mL. Then the experiment continued observed in different agitation rate at 50, 100, 150, 200, 250 and 300 rpm. After overall observation, the best range of inoculum size gave between 0.8 mL to 1.2 mL and the range of agitation rate was identified between 230 rpm to 270 rpm. The best range of culture conditions was used for further study using Respond Surface Methodology (RSM). The optimal set of culture conditions for high soluble recombinant chitinase was determined as an inoculum size of 0.98 mL and 244.7 rpm of agitation rate. From the experimental value, the optimal production of recombinant chitinase was achieved 0.376 U/mL. This shows the 1.57-fold increment compared to the initial experiment which produced 0.239 U/mL of chitinase activity. These conditions successfully enhance the production of chitinase by using recombinant protein in Escherichia coli. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Chitinase |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Escherichia coli |