MARC details
| 000 -LEADER |
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| fixed length control field |
02736nam a2200265 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000045194 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204422.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
100225t2009 my a f m 000 0 eng|d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004503(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905170944 |
| Level of effort used to assign nonsubject heading access points |
rohana |
| Level of effort used to assign subject headings |
201108030938 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201107132308 |
| Level of effort used to assign classification |
VLOAD |
| -- |
201002251400 |
| -- |
ida |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP248.65.E59 2009 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nur Azhar Saari |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology/ |
| Statement of responsibility, etc. |
Nur Azhar Saari |
| 246 3# - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
Optimization of temperature and agitation rate on expression of recombinant xylanase in Kluyveromyces lactics using response surfacemethodology |
| Medium |
[computer file] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2009 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiii, 47 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 computer disc |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 35-39 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Xylanase has been used widely in industries today due to its ability in catalyzing various types of biochemical reactions. However, production cost of xylanase enzyme is very expensive. A research to reduce the cost by optimization of temperature and agitation rate on expression of recombinant xylanase in K. lactis using Response Surface Methodology (RSM) was successfully done. At the early stage of experiment, one factor at a time was employed to screen the best range for temperature and agitation rate. All the parameter ranges obtained were used in Response Surface Methodology (RSM). Response Surface Methodology in Design Expert version 7.1.6 software was used with Central Composite Design (CCD) mode. Thirteen sets of experiments with different parameter values were suggested by the software. The predicted optimum values for temperature, agitation rate and xylanase activities were 34.68oC, 253 rpm and 9.198 U/ml respectively. One set of experiment was run using the optimized parameter and as a result, 9.221 U/ml xylanase activity was recorded. Before optimization, xylanase activity was only 5.221 U/ml and the activity was increased by 43.75% or 1.78-fold after optimization. The optimization also reduces the energy consumption as the temperature was reduced from 38oC to 34.6oC and agitation rate reduced from 280 to 253 rpm. As conclusion, this research is successful to increase recombinant xylanase production, reduce the energy consumption and also able to reduce the production cost.-Author- |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Xylanases |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enzymes |
| General subdivision |
Biotechnology |