MARC details
| 000 -LEADER |
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| fixed length control field |
02542nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000045481 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204424.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
100430t2009 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002186(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131712 |
| Level of effort used to assign nonsubject heading access points |
yusri |
| Level of effort used to assign subject headings |
201112051409 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201108030912 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201107132316 |
| Level of effort used to assign classification |
VLOAD |
| -- |
201004301516 |
| -- |
ida |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QD702.C5 N67 2009 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Norheziwan Hamsah |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology / |
| Statement of responsibility, etc. |
Norheziwan Bin Hamsah |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2009 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiv, 56 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 computer disc |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. [42]-49 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The function of chitinase as a chitin digestive enzyme has attracted the attention as one of the potential materials for control of phytopathogenic fungi and insect pests. However, chitinase is uneconomic for commercialized due to the high cost in its production. The development in recombinant DNA technology has helped to reduce chitinase production cost and enhance its production. The aim of this study is to determine the optimum conditions toward the production of recombinant chitinase in Escherichia coli by manipulating the concentration of inducers (IPTG and lactose). The best concentration of IPTG and lactose as inducers were determined by using the conventional method. Results from conventional method were used for further improvement of optimization by using Response Surface Methodology (RSM). In Response Surface Methodology, the optimization of expression was carried out based on the Central Composite Design (CCD). The effect of the inducers concentration toward the expression level of the recombinant chitinase production were analyzed and optimum values of tested variables for the production of chitinase were 1.19 mM IPTG and 0.98% lactose. Response surface analysis revealed that 0.513 U/ml of chitinase activity was achieved by using the optimized condition. This improved about 13.2-fold compare to initial experiment which produced 0.039 U/ml. This showed that combination between IPTG and lactose has increased the production of recombinant chitinase. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Chitin |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Response surfaces (Statistics) |