Extraction of papain enzyme from papaya leaves using enzyme assisted method / (Record no. 2181)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02401nam a2200253 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000056459 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204439.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 111201t2010 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0004355(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905161046 |
| Level of effort used to assign nonsubject heading access points | rohana |
| -- | 201112010842 |
| -- | Fida |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TP248.E5 R67 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Roswani Shaimi |
| 245 10 - TITLE STATEMENT | |
| Title | Extraction of papain enzyme from papaya leaves using enzyme assisted method / |
| Statement of responsibility, etc. | Roswani Shaimi |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2010 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xii, 61 p. : |
| Other physical details | ill. (some col.) ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 computer disc |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 49-52 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase assisted extraction process was optimized by varying different parameters such as pH of extraction process, Cellulase concentration, Solid to Liquid ratio, incubation time and incubation temperature. Each 5 g of papaya leaves were ground and mixed with acetate buffer at a different Solid to Liquid ratio (1:5 - 1:25 g/ml) and adjusted with different pH (3-8). Cellulase was quantified and dispersed in acetate buffer (Na2HPO—citric acid) in different concentration (0.5 - 2.5 mg/ml).The enzymatic pretreatment was carried out and continued for enzyme assisted extraction process for various temperature and time (5070o4C and 1-5 h respectively). The optimal extraction conditions that satisfied the above constraints were found to be at pH 7, 1.5 mg/ml of Cellulase, solid to liquid of 1:10 (g/ml), an extraction time of 4 h and at an extraction temperature of 65oC. Under the optimum conditions, the extraction yield of papain was successfully achieved as much as 3.8018 µmole with 2.0910 Units/ml in Cellulase treated sample. Therefore, the potential for the papain enzyme in medical field can be fulfilled using enzyme assisted extraction. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzymes |
| General subdivision | Biotechnology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Extraction (Chemistry) |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TP248.E5 R67 2010 rs Bc. | 0000060250 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 5512 | TP248.E5 R67 2010 rs Bc. | 0000060251 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |