Adsorption study of fructosyltransferase immobilization by using various adsorbent / (Record no. 2471)

MARC details
000 -LEADER
fixed length control field 02877nam a2200265 a 4500
001 - CONTROL NUMBER
control field vtls000075800
003 - CONTROL NUMBER IDENTIFIER
control field KUKTEM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20251114204449.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 131030t2013 my da f abm 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0003823(Local)
039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE]
Level of rules in bibliographic description 201905151525
Level of effort used to assign nonsubject heading access points shah
Level of effort used to assign subject headings 201905151525
Level of effort used to assign classification shah
-- 201310301653
-- nabilah
040 ## - CATALOGING SOURCE
Original cataloging agency UMP
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) TP156.A35 H39 2013 rs Bc.
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Nor Hazwani Syuhada Azmi
245 10 - TITLE STATEMENT
Title Adsorption study of fructosyltransferase immobilization by using various adsorbent /
Statement of responsibility, etc. Nor Hazwani Syuhada Azmi
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Kuantan, Pahang :
Name of publisher, distributor, etc. UMP,
Date of publication, distribution, etc. 2013
300 ## - PHYSICAL DESCRIPTION
Extent xviii, 115 p. :
Other physical details ill. ;
Dimensions 30 cm. +
Accompanying material 1 CD-ROM
502 ## - DISSERTATION NOTE
Dissertation note Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Bibliography : p. 100-106
520 3# - SUMMARY, ETC.
Summary, etc. Immobilized enzyme are widely used in the industry because of its stability and economic. Because of the high demand from the industry, the research is done in order to enhance the quality of immobilized enzyme. This study discusses about the adsorption in the enzyme immobilization process. The purposes of this study are to find out the species of sawdust (Keruing, Meranti and Kempas) which have the excellent adsorption capability, to investigate the effect of various initial enzyme concentrations and the time required for the adsorption of adsorbate onto adsorbent reach equilibrium. For the immobilization process, it was occurred in water bath shaker for 26 h at room temperature and at pH 5.5. The enzyme activity was analyzed by using DNS method. To determine the equilibrium time, the solutions was being agitated in water bath shaker at 100 rpm and the samples were taken out at a given time intervals for analyses (0-40 hours). The concentration of FTase was analyzed by using UV-Vis Spectrometer. The effect of various initial enzyme concentrations (0, 2, 4, 6, 8, 10, 12, and 14 g/L) in the adsorption process was analyzed by using UV-Vis Spectrometer and employing Langmuir and Freundlich models in order to find out the isotherm which fit with the data obtained.SEM and FTIR was done to identify the structures of the untreated, treated sawdust and immobilize enzyme. All the three adsorbents had achieved the equilibrium at time 25 to 30 hours and it is showed that Keruing adsorbent comes out with the highest loading capacity (0.945g of FTase/L) due to the size of pores itself. Enzyme concentration residue increases after the addition of 6 g/L of FTase due to the amount of enzyme were exceeds the amount of adsorbent. Freundlich isotherms fitted the data while Keruing showed the highest capability of adsorption followed by Meranti as adsorbent.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Adsorption
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Immobilized enzymes
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Wood waste
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not lost   Not for loan UMPLIB GAMBANG UMPLIB GAMBANG 04/09/2019   TP156.A35 H39 2013 rs Bc. 0000076141 04/09/2019 1 04/09/2019 Final Year Report
  Not lost   Not for loan UMPLIB GAMBANG UMPLIB GAMBANG 04/09/2019   CD 7332 | TP156.A35 H39 2013 rs Bc. 0000076142 04/09/2019 1 04/09/2019 Final Year Report

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