MARC details
| 000 -LEADER |
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| fixed length control field |
02515nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000045098 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204453.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
100205t2009 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004502(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905170943 |
| Level of effort used to assign nonsubject heading access points |
rohana |
| Level of effort used to assign subject headings |
201108030913 |
| Level of effort used to assign classification |
Fida |
| Level of effort used to assign subject headings |
201107132306 |
| Level of effort used to assign classification |
VLOAD |
| Level of effort used to assign subject headings |
201002081446 |
| Level of effort used to assign classification |
kam |
| -- |
201002051509 |
| -- |
kam |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP248.65.C92 U46 2009 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Ummi Nor Husna Husin |
| 245 10 - TITLE STATEMENT |
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| Title |
Partial characterization of cyclodextrin glucanotransferase by Bacillus sp. TS1-1 / |
| Statement of responsibility, etc. |
Ummi Nor Husna Husin |
| 246 3# - VARYING FORM OF TITLE |
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| Title proper/short title |
Partial characterization of cyclodextrin glucanotransferase by Bacillus sp. TS1-1 |
| Medium |
[computer file] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2009 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
37 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 computer disc |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The crude culture that contains cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) from Bacillus sp. TS1-1 has been partially purified by centrifugation and cross-flow filtration. Initial 242.74 U/ml of CGTase was detected in the culture after 24 hours of incubation. The crude supernatant obtained after centrifugation for 5000rpm, 5 minutes and 4oC was subsequently filtered at 15oC through cross-flow filtration using Kvick Lab cross-flow system. Two cassettes were used with molecular weight cut off of 50K and 10K. The retentate from 50K cassette was further filtered through 10K cassette. Each permeate and retentate from each cassette were tested for CGTase activity. Using cross-flow filtration, the crude enzyme was purified 2.33 fold. The crude and partially purified enzyme was then subjected to gel electrophoresis (SDS-PAGE) to determine the enzyme molecular weight. The partial purified enzyme especially at retentate 50K suggested an initial size of CGTase between 46 to 88 kDa. The crude and partial purified CGTase was then assayed using phenolpthalein method with slight modification for the determination of CGTase optimum pH, pH stability, optimum temperature and thermal stability. Based on this work, the optimum temperature for activity was at 40oC and 80oC, the thermal stability was from 40-70oC and from 60-90oC; the optimum pH for activity was at pH6 and pH10; the pH stability was from pH4 to 6. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Cyclodextrins |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Enzymes |
| General subdivision |
Purification |