Extraction of papain enzyme from papaya leaves using hot water extraction with ultrasonic – assisted pretreatment / (Record no. 2982)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02197nam a2200253 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000056480 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204507.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 111201t2010 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0004351(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905161041 |
| Level of effort used to assign nonsubject heading access points | rohana |
| -- | 201112011121 |
| -- | Fida |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TP248.E5 I99 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Izzah Suhaila Abu Bakar |
| 245 10 - TITLE STATEMENT | |
| Title | Extraction of papain enzyme from papaya leaves using hot water extraction with ultrasonic – assisted pretreatment / |
| Statement of responsibility, etc. | Izzah Suhaila Abu Bakar |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2010 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiii, 68 p. : |
| Other physical details | ill. (some col.) ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 computer disc |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. [51]-56 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Papaya leaf is not just a byproduct of papaya tree but it contains an essential enzyme which is papain. The aim of the study is to measure the amount of this enzyme extracted from leaf tissue using hot water extraction with ultrasonic-assisted pretreatment as well as to determine the optimum condition for the extraction process. The leaves were subjected to a pretreatment which is grinding and ultrasonication followed by hot water extraction and finally, enzymatic analysis using UV-Vis spectrophotometer. The parameters involved in the process were ultrasonication time (30 – 90 minutes) and frequency (18 – 26 kHz), solid-solvent ratio (1:5 – 1:25), and extraction time (1 – 5 hour) and temperature (50 - 70°C). Among these parameters, maximum concentration of papain enzyme was achieved at 60 minutes and 20 kHz of ultrasonication, solid-solvent ratio at 1:5, extraction time at 4 hours and extraction temperature at 60°C. In conclusion, the maximum concentration of papain enzyme achieved at this optimum parameter was 4.020 µM. Utilization of PEG–phosphate system for separation and purification of papain is recommended in order to get the pure papain enzyme. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzymes |
| General subdivision | Biotechnology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Extraction (Chemistry) |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TP248.E5 I99 2010 rs Bc. | 0000060274 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 5525 | TP248.E5 I99 2010 rs Bc. | 0000060275 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |