Whey protein fractionation based on sp-sepharose cation exchange chromatography / (Record no. 2999)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02022nam a2200241 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000058052 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204507.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 111227t2010 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0004142(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905132127 |
| Level of effort used to assign nonsubject heading access points | farhana |
| -- | 201112271521 |
| -- | Fida |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TP156.F5 H39 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hazman Abd Mulok |
| 245 10 - TITLE STATEMENT | |
| Title | Whey protein fractionation based on sp-sepharose cation exchange chromatography / |
| Statement of responsibility, etc. | Hazman Abd Mulok |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2010 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiv, 50 p. : |
| Other physical details | ill. (some col.) ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: p. 40-41 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Bovine whey protein consists of different type of proteins such as βlactoglobulin (β-lag), α-lactalbumin (α-lac), immunoglobulin, bovine serum albumin (BSA), lactoferrin, lactoperoxidase and glycomacropeptide. Single protein is more valuable compare to the mixture of proteins and it can be used for specific application. The main objective of this study is to fractionate whey protein components into several groups or single protein fraction using SP-sepharose TM Fast Flow (Amersham Biosciences) cation exchange chromatography column. The chromatographic process was run at different pH range from pH 4 to pH 8 using 24 ml column. All major whey proteins were recovered in the elution fraction at pH 4 and pH 4.5. No protein was bound at pH 7 and pH 8. The best whey fractionation from 2 ml whey feed was achieved at pH 4 with the yield for β-lag, BSA and α-lac are 83.33%, 66.44% and 10.51% respectively. Different protein fraction recovered during fractionation process at different pH using cation exchange chromatography process, can be used as a guideline to isolate particular protein of interest from bovine whey. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Ultrafiltration |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TP156.F5 H39 2010 rs Bc. | 0000060951 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 5640 | TP156.F5 H39 2010 rs Bc. | 0000060952 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |