Protein purification by using immobilied metal ions affinity zeolite adsorbent / (Record no. 3110)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02360nam a2200241 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000058594 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204511.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120306t2010 my a f m 001 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0004536(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905311531 |
| Level of effort used to assign nonsubject heading access points | nazirah |
| Level of effort used to assign subject headings | 201905131604 |
| Level of effort used to assign classification | azhar |
| -- | 201203061335 |
| -- | ida |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TP248.65.P76 S86 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Su Najwa Mohamed |
| 245 10 - TITLE STATEMENT | |
| Title | Protein purification by using immobilied metal ions affinity zeolite adsorbent / |
| Statement of responsibility, etc. | Su Najwa Mohamed |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2010 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xv, 76 p. : |
| Other physical details | ill. (some col.) ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. [59]-62 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The applications for purified proteins have grown considerably over recent years due to their biological and nutritional properties. The aim of this research are to develop zeolite as inorganic stationary substrate in immobilized metal ion affinity (IMA) for protein purification and to study the optimum condition to purify protein in order to maximize yield and purity of protein by applying the principle of adsorption process. The effect of contact time, different types of adsorbent and various pH values were studied in a batch experiment by using Bovine Serum Albumin (BSA) as protein sample. The sorption equilibrium was reached within 10 minutes. Fe3+ ion showed the best performance for BSA and has the suitable interaction with both HY and HBeta zeolite framework and the protein as compared to Pb2+, Ni2+ ions. The adsorption capacity for BSA was found to be the highest at pH 4.5 that is close to isoelectric point (pI) with Fe- HBeta used as adsorbent. The adsorbed amount increases as the pH increases and reduces as the pH exceeds the pI value. This result proves that adsorption capacity of Bovine Serum Albumin depends on the types of adsorbent used, which means the adsorption stoichiometries depends on physical characteristic of adsorbent that could increase and reduce the adsorption capacity of the protein as well as the pH of protein. The adsorption isotherm data of BSA is well fitted to the Langmuir isotherm model. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Protein |
| General subdivision | Purification |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TP248.65.P76 S86 2010 rs Bc. | 0000063066 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 5808 | TP248.65.P76 S86 2010 rs Bc. | 0000063067 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |