The extraction of papain from papaya leaves / (Record no. 3119)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01949nam a2200253 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000058544 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204512.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120302t2010 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0004348(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905161036 |
| Level of effort used to assign nonsubject heading access points | rohana |
| Level of effort used to assign subject headings | 201203021211 |
| Level of effort used to assign classification | Fida |
| -- | 201203021210 |
| -- | Fida |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TP248.E5 D53 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Nur Dianaty Nordin |
| 245 14 - TITLE STATEMENT | |
| Title | The extraction of papain from papaya leaves / |
| Statement of responsibility, etc. | Nur Dianaty Nordin |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2010 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xv, 45 p. : |
| Other physical details | ill. (some col.) ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 38-41 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The use of papaya leaves to extract papain enzyme was evaluated for different extraction technique mainly using grinding method, enzyme-assisted extraction and ultrasonication. Enzyme concentration was then determined by measuring the amount of amino acid released in the reaction of protease assay. The combination of grinding method, enzyme-assisted extraction was proven to give highest yield of papain by 36.75% when compared to grinding alone. Then further purification of the extracted papain was done by using ammonium sulfate precipitation. The optimized concentration of ammonium sulfate saturation was achieved at 55% concentration in order to fully precipitate the protein. The combination of grinding, enzyme-assisted extraction and ultrasonication can be considered as a potential extraction technique to extract papain as it is more delicate towards protein extraction. Furthermore, the investigation on other source of papain such as from the petioles, stalks and stem could also be done. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzymes |
| General subdivision | Biotechnology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Extraction (Chemistry) |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TP248.E5 D53 2010 rs Bc. | 0000063285 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | In Transit | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 5957 | TP248.E5 D53 2010 rs Bc. | 0000063286 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |