MARC details
| 000 -LEADER |
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| fixed length control field |
02641nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000058608 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204514.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
120308t2010 my a f m 001 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002050(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131619 |
| Level of effort used to assign nonsubject heading access points |
yusri |
| Level of effort used to assign subject headings |
201203080816 |
| Level of effort used to assign classification |
ida |
| -- |
201203080815 |
| -- |
ida |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QC609.C39 F37 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mohd Faris Mohd Yunus |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of medium formulation on production of recombinant chitinase in Escherichia coli / |
| Statement of responsibility, etc. |
Mohd Faris Mohd Yunus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2010 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiii, 46 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 40-44 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Chitinase is enzyme that hydrolyzing chitin to produce N-acetyl- glucosamine. Chitinase can be found in bacteria, fungi, higher plant, insect and some vertebrates. There are a lots of applications of chitinase as the demands of the enzyme is rising high in the market due to usage in pharmaceutical, biopesticides or food industry. For this study, chitinase enzyme was expressed in recombinant bacteria using Escherichia coli as a host. The effect of various medium on expression of chitinase in Escherichia coli was conducted in this research. Five medium were studied. LB, TB, SB, SOB and 2x YT were screened. LB medium gave the best result of highest chitinase enzyme activity. Each component of LB medium was study to determine the optimize amount of composition to produce the highest enzyme activity. The best range of every composition obtained from the conventional method was 3.0 g/l to 7.0 g/l for sodium chloride, 1.5 g/l to 4.5 g/l for yeast extract and 10 g/l to 14 g/l for tryptone. The optimization was done using response surface methodology (RSM) conducted by software named Design Expert. The optimal medium composition for high soluble recombinant chitinase was determined as 3.63 g/l of sodium chloride, 4.50 g/l of yeast extract and 13.11 g/l of tryptone. From the experimental, the enzyme activity after optimization was achieved 2.291 U/ml compared to predicted response of 2.409 U/ml. This result shows increment of the activity for 82% than before the optimization which is 0.411 U/ml. It shows that the optimization of the medium formulation to improve the expression and production of chitinase was successfully conducted by using RSM. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Chitinase |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Escherichia coli |