MARC details
| 000 -LEADER |
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| fixed length control field |
02404nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000058774 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204517.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
120327t2010 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004533(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131550 |
| Level of effort used to assign nonsubject heading access points |
azhar |
| Level of effort used to assign subject headings |
201203270925 |
| Level of effort used to assign classification |
Fida |
| -- |
201203270925 |
| -- |
Fida |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP248.65.P76 M39 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mohd Mazlan Mohamed |
| 245 10 - TITLE STATEMENT |
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| Title |
Isolation Of ß-Lactoglobulin and a-Lactalbumin from whey / |
| Statement of responsibility, etc. |
Mohd Mazlan Mohamed |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2010 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xii, 39 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. [30]-32 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Whey is a byproduct in cheese production. Whey solution is rich with proteins; the main proteins in whey are β-Lactoglobulin (β-Lg) (50%), α-Lactalbumin (α-La) (20%), bovine serum albumin (BSA) (10%) and immunoglobulin’s (Igs) (10%). Single protein has higher value then whey solution and has specific application. Hence this paper aims to isolate two of main whey proteins that are β-Lg and α-La due to their functions and higher value of pure protein. In this work, whey was prepared during casein coagulation from skim milk by acidic precipitation at pH below than 5 with 0.5 M hydrochloric acid. The experiments were conducted by AKTA Fast Protein Liquid Chromatography (FPLC), using size exclusion (packed by superdex TM 75 gel filtration media in an empty size exclusion XK column (60 ml)) and cation exchange column (HiTrap SP HP, 1ml column) in order to isolate both proteins from whey and 10% separating gel of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) was used to identify each protein form isolation steps and to determine the degree of purity of β-Lg and α-La. Silver staining was used in developing protein band on SDS gel. The experimental results concluded that both β-Lg and α-La can be isolated from other proteins in whey by using size exclusion and cation exchange fast protein liquid chromatography. Both β-Lg and α-La were visualized at 18 kDa and 14 kDa respectively, in SDS-PAGE. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Proteins |
| General subdivision |
Separation |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Separation (Technology) |