MARC details
| 000 -LEADER |
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| fixed length control field |
02335nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000068064 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204523.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
130109t2012 my a f m 000 0 eng|d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0003879(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905161515 |
| Level of effort used to assign nonsubject heading access points |
shah |
| Level of effort used to assign subject headings |
201302041534 |
| Level of effort used to assign classification |
huda |
| Level of effort used to assign subject headings |
201302041532 |
| Level of effort used to assign classification |
huda |
| Level of effort used to assign subject headings |
201301091648 |
| Level of effort used to assign classification |
Fida |
| -- |
201301091558 |
| -- |
Fida |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP156.C5 A45 2012 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nurul Alia Khalil |
| 245 10 - TITLE STATEMENT |
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| Title |
Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer / |
| Statement of responsibility, etc. |
Nurul Alia Khalil |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2012 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiii, 43 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2012 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 33-35 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Nowadays, with the increase demand of protein production and purity, the purification cost of the protein also increases about 50 to 90%. Packed bed chromatography is widely used in protein separation. However, there are some limitations of the packed bed chromatography such as high-pressure drop, channeling problem and complicated scale up process. Most of these limitations can be overcome by membrane chromatography. The main objective of this research is to prepare cation-exchange membrane chromatography from polyamide membrane by chemical grafting of methacrylic acid monomer. Potassium persulfate and potassium metabisulfite was used to generate the radicals in the polyamide membrane and grafted with methacrylic acid monomer together with ethylene glycol dimethacrylate as a cross-linker. Different monomer concentration between 0.1 and 1.0M and monomer grafting duration from 15 – 120 min was studied toward producing high protein binding capacity membrane. Optimum time for grafting methacrylic acid onto polyamide membrane was 45 minutes with the binding capacity 599.6390 mg/g BSA at the monomer concentration of 0.1 M. Identification and optimization of critical parameter in grafting process is crucial for the development of high performance membrane chromatography materials. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Chromatography |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Chromatography |
| General subdivision |
Analysis |