MARC details
| 000 -LEADER |
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| fixed length control field |
02557nam a2200241 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000067954 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204528.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121226t2012 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004510(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131428 |
| Level of effort used to assign nonsubject heading access points |
azhar |
| -- |
201212261604 |
| -- |
Fida |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP248.65.E59 S93 2012 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Muhamad Syafiq Eidham Ab Rashid |
| 245 10 - TITLE STATEMENT |
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| Title |
Production of extracellular tannase enyzme by Aspergillus Niger / |
| Statement of responsibility, etc. |
Muhamad Syafiq Eidham Ab Rashid |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2012 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiv, 58 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2012 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography: p. 51-54 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Tannase catalyses the hydrolysis reaction of the ester bond present in gallic acid esters and hydrosable tannins. This enzyme is produced by plants and microorganism and used in food industry such as tea industry. In order to produce high yields of tannase enzymes, it needs optimization of parameters which normally consuming a lot of time and expensive to be optimized conventionally. In this study, Response Surface Methodology (Central Composite Design) was performed for optimization. Aspergillus niger had been chosen as biomass and glucose as substrate in this study. The fermentation study was take place in the shake flask and was optimized for higher tannase enzyme activity. There are three factors was studied in this study which are the pH of the fermentation medium (pH 3.5-pH 6.0), the substrate concentration (2 w/v%-10 w/v%) and the agitation speed (100 rpm-300 rpm). From one factor at a time (OFAT) analysis, tannase enzyme showed that the optimum tannase activity at pH 5, 6.0 w/v% and 200 rpm with 14.9521 U/ml, 13.1256 U/ml and 12.4301 U/ml. RSM results shows that the optimum values of each parameter were pH 4.75, 6.0 v/w% of substrates concentration and 200 rpm of agitation speed which resulted the optimum tannase activity at 15.3131 U/ml. As a conclusion, RSM is the best tool used to identify the correlation between controlled independent factors and observed dependent responses. For the future research, it is recommended for study the application of genetic engineering in the enzyme production by scale up tannase production using a bioreactor and toxicology studies on tannase enzyme for application in food industry. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Enzymes |
| General subdivision |
Biotechnology |