Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer / (Record no. 4034)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02444nam a2200241 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000075750 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204543.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 131028t2013 my da f abm 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0002098(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905131634 |
| Level of effort used to assign nonsubject heading access points | yusri |
| -- | 201310281041 |
| -- | nabilah |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | QD79.C453 Y84 2013 rs Bc. |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Yue, Wei Lee |
| 245 10 - TITLE STATEMENT | |
| Title | Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer / |
| Statement of responsibility, etc. | Yue Wei Lee |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2013 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xvii, 79 p. : |
| Other physical details | ill. ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 57-60 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involve during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). Then, the membrane was immersed in diamine solution of 1,2-diaminoethane or 1,4-diaminobutanhe to produce positively charged membrane chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding capacity of 0.385±0.027mgBSA/cm2 membrane. Based on FTIR transmission peak, both N-H and C-N functional groups were detected in modified membrane that indicated the successful of grafting process. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Ion exchange chromatography |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | QD79.C453 Y84 2013 rs Bc. | 0000074681 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 7040 | QD79.C453 Y84 2013 rs Bc. | 0000074682 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |