MARC details
| 000 -LEADER |
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| fixed length control field |
02352nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000076087 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204546.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
131113t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002261(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905241130 |
| Level of effort used to assign nonsubject heading access points |
shamsul |
| -- |
201311131239 |
| -- |
nabilah |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QP609.S47 S93 2013 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Siti Syarrah Mohammad Hanapiah |
| 245 10 - TITLE STATEMENT |
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| Title |
Stability and kinetic study of serine protease from exocarp of Benincasa Hispida / |
| Statement of responsibility, etc. |
Siti Syarrah Mohammad Hanapiah |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2013 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xviii, 39 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. xix-xxii |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Serine protease is widely used in many industrial applications especially in food industry because of their wide range of good solubility, substrate specificity, activity over wide pH and temperature range and high stability under extreme conditions. Benincasa hispida contains serine protease that usually can be found in the sarcocarp or seeds. Therefore, exocarp of Benincasa hispida is used determine the presence of serine protease. This research objective was to investigate the effect of pH, temperature and stability of the enzyme activities and also the enzyme kinetic of serine protease. Serine protease from exocarp of Benincasa hispida is purified using ammonium sulfate precipitation, and its activity at different parameters were measured by using ultraviolet-visible spectroscopy (uv-vis) at wavelength 660nm. The activity was inhibited by trichloroacetic acid, CCl3 COOH. The optimum pH for their activity is in alkaline range (pH7 – 9), and the enzyme activity dropped at pH11. Meanwhile, the optimum temperature of serine protease is in the range 50oC to 60oC, where its activity started to slows down at 70oC. The serine protease had a Michaelis-Menten constant, KM of 0.05172 mg/ml whereas the maximum rate of reaction, VMAX is . Serine protease activity is determined by the amount of enzyme that hydrolyzed casein to produce 1.0 µmole of tyrosine per minute. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Serine proteinases |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Cucurbitaceae |