MARC details
| 000 -LEADER |
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| fixed length control field |
03111nam a2200265 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000075808 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204546.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
131031t2012 my da f abm 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002217(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905241018 |
| Level of effort used to assign nonsubject heading access points |
shamsul |
| -- |
201310311118 |
| -- |
nabilah |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QK495.Z65 M45 2012 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mellisa Abdullah |
| 245 10 - TITLE STATEMENT |
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| Title |
Production and characterization of protein extract from lempoyang ginger / |
| Statement of responsibility, etc. |
Mellisa Abdullah |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2012 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xii, 40 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 34-35 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Antimicrobial protein has received tremendous attention especially by the pharmaceutical industry, which sources mainly extracted from nature such as herbs and plants. These sources are known for their nontoxic property, biodegradable, as well as easily available in the market. Among the numerous natural resources, the Lempoyang ginger which scientifically known as Zingiber zerumbet L. Smith had long known for its application as antifungal, anti-inflammatory, anti-ulceration and antioxidant. It is also known for treating diarrhea, coughs, asthma and some other skin diseases. Lempoyang ginger is selected for this research as very few information was published on protein of this Zingiberaceae family of which among all, zerumbone was widely extracted as the major component. This research aimed to produce the protein extract from Lempoyang using the expanded bed adsorption chromatography (EBAC) and then characterize it using the sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). The parameter that was varied for this research is the height of the bed settlement at 5cm, 6cm, 9cm and 11cm, with elution made in two-steps using 45% ethanol and 90% ethanol buffer solutions. Amberlite XAD7HP is used as adsorbents to bind the large complex protein molecules from Lempoyang feedstock with constant pump speed at 13rpm for each cycle. The eluted protein fractions are then subjected to Lowry‟s protein concentration determination technique before characterized for molecular weight determination using the SDS-PAGE tools. The dynamic binding capacity is determined from the 50% breakthrough of the initial feedstock solution, and the recovery ratio is determined for total protein obtained. From the results, protein concentration is higher when eluted with 45% ethanol buffer solution as protein were loosely bounded to the adsorbents while the first elution using 90% ethanol buffer solution showed a lower protein concentration. The washed protein and eluted protein fractions from EBAC which were tested on SDS-PAGE showed a protein band of eluted fraction protein band with molecular weight of 21.12 kDa. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Zingiberaceae |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Anti-infective agents |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Extraction (Chemistry) |