MARC details
| 000 -LEADER |
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| fixed length control field |
02402nam a2200241 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000075157 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204550.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
131008t2012 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002205(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131718 |
| Level of effort used to assign nonsubject heading access points |
yusri |
| Level of effort used to assign subject headings |
201311181656 |
| Level of effort used to assign classification |
nabilah |
| Level of effort used to assign subject headings |
201310221704 |
| Level of effort used to assign classification |
nabilah |
| -- |
201310081219 |
| -- |
nabilah |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QK495.C74 H39 2012 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nor Hayati Shahidan |
| 245 10 - TITLE STATEMENT |
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| Title |
Extraction of stevioside from Stevia Rebaudiana leaves using cellulase / |
| Statement of responsibility, etc. |
Nor Hayati Shahidan |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2012 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiv, 49 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 38-42 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Stevioside is a diterpene glycoside present in Stevia Rebaudiana leaves that has the ability to sweeten at rated between 70 to 350 times than sucrose (0.4% w/v). It has no calorific value. Unlike many low calorie sweeteners, stevioside is stable at high temperature. The objective of this research is to extract stevioside from stevia rebaudiana leaves by using cellulase from Aspergillus Niger. Acetate buffer and ethanol were used as a medium for enzyme and as a solvent, respectively. In this present study, the enzymatic extraction of stevioside from stevia rebaudiana leaves was carried out using cellulase with various parameters that affect the production of stevioside such as concentration of enzyme, incubation time and temperature. Cellulase was observed to give the highest stevioside yield (16230 ± 0.3 μg/ml) at 40oC. This indicated that the maximum temperature for cellulase activity was 40oC. The results signify that the enzymatic extraction method is an alternative to solvent based stevioside extraction, based on its higher efficiency. Thus, it can be concluded that the extraction of stevioside from Stevia rebaudiana leaves using cellulase can be maximized under the maximum conditions for the cellulase activity where the used of solvent can be minimized in degrading the cell wall Together with the maximum heat and correct combination of the solvent used, a new and efficient way of extracting high yield of stevioside can be obtained. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Stevia rebaudiana |