MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02305nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000076092 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20251114204550.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
131113t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
THE0002097(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
|---|
| Level of rules in bibliographic description |
201905131634 |
| Level of effort used to assign nonsubject heading access points |
yusri |
| -- |
201311131511 |
| -- |
nabilah |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QD79.C453 C43 2013 rs Bc. |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chan, Fong Shiew |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Development of multiple interactions mixed matrix membrane chromatography using lewatit MP500 anion resin and lewatit CNP 105 cation resin for whey protein fractionation / |
| Statement of responsibility, etc. |
Chan Fong Shiew |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2013 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xv, 51 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Bibliography : p. 45-50 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
The conventional method to purify protein is by using packed bed column chromatography. However, this method had several limitations such as high pressure drop and limited flow rate operation. Membrane chromatography can be used to overcome the limitation of packed column but the preparation of adsorptive membrane requires harsh chemical modifications. Mixed matrix membrane (MMM) preparation concept can be used as an alternative route to prepare membrane chromatography by physical blending of adsorptive resin with membrane polymer solution. In the current research, multiple interactions MMM chromatography was developed for whey protein fractionation using 7.5 wt% CNP105 cation resin and 42.5 wt% MP500 anion resin relative to base polymer content. The resins were blend at different composition in EVAL and cellulose base polymer matrix. Based on HPLC and SDS-PAGE analysis, both acidic and basic whey proteins were bound to the multiple interactions MMM in single run of whey batch fractionation. The binding capacity for major acidic whey proteins using EVAL based MMM are 4.255 mg BSA bound/ g MMM, 60.887 mg α-Lac bound/ g MMM and 231.788 mg β-Lac/ g MMM. For CA based MMM, the binding capacity are 2.970 mg BSA bound/ g MMM, 42.392 mg α-Lac bound/ g MMM and 179.817 mg β-Lac/ g MMM. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Ion exchange chromatography |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Whey |