Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography / (Record no. 4251)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02402nam a2200265 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000076023 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204551.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 131112t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0005052(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905140937 |
| Level of effort used to assign nonsubject heading access points | SHAHRILJ |
| -- | 201311121408 |
| -- | nabilah |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TX377 .R33 2013 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Rabiatul Adawiyah Sharom |
| 245 10 - TITLE STATEMENT | |
| Title | Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography / |
| Statement of responsibility, etc. | Rabiatul Adawiyah Sharom |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2013 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiii, 49 p. : |
| Other physical details | ill. ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 45-47 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | In dairy industry, whey protein fractionation is an important process that requires an effective method to separate valuable proteins in bovine whey protein. Bovine whey protein can be divided into two components which are major protein and minor proteins. The major protein contains approximately 50% of β-lactoglobulin (β-Lg), 20% of α-lactalbumin (α-Lac), 10% of bovine serum albumin (BSA) and 10% of immunoglobulin (Ig). Cation exchange and anion exchange chromatography is one of advance separation techniques that can fractionate bovine whey protein. All negatively charged proteins in whey were bound simultaneously to an anion exchange column (Q-Sepharose), while all positively charged proteins in whey were bound to a cation exchange column (SP-Sepharose). Whey protein and buffer solution (pH 4 - pH 10) were prepared before run the experiment. The method is based on the use of an ionic column and salt gradient elution buffer (Buffer solution plus 1 M NaCl). The collected fractions were analyzing using SDS-PAGE to determine which fractions that contain protein component. By using UPLC the concentration of protein exists in the fraction had been determined. Protein fractionation at pH 7 gives the best result with highest concentration of protein recover by using Q-Sepharose. While, by using SP-Sepharose show pure β-Lg can be fractionate at pH 5. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Whey products |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Ion exchange chromatography |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chromatographic analysis |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TX377 .R33 2013 rs Bc. | 0000074665 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 7032 | TX377 .R33 2013 rs Bc. | 0000074666 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |