Enzymatic extraction of stevioside from Stevia Rebaudiana leaves using cellulase / (Record no. 4287)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02264nam a2200253 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000076030 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204553.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 131112t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0002227(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905241039 |
| Level of effort used to assign nonsubject heading access points | shamsul |
| -- | 201311121626 |
| -- | nabilah |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | QK896 .N37 2013 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Nasihah Mohd Nor |
| 245 10 - TITLE STATEMENT | |
| Title | Enzymatic extraction of stevioside from Stevia Rebaudiana leaves using cellulase / |
| Statement of responsibility, etc. | Nasihah Mohd Nor |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2013 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xv, 40 p. : |
| Other physical details | ill. ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 34-35 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Stevioside is one of the components known as diterpene glycoside existing in Stevia Rebaudiana leaves which have 250 to 300 sweeter than sucrose at the concentration of 0.4% (w/v). It is used as sweetening agents and taste modifier mostly in food industry. Moreover, stevioside has no calorific value and suitable used in therapeutic especially in treating diabetic people. The objective of this research is to extract the stevioside from Stevia rebaudiana leaves by using cellulase from Aspergillus Niger.The medium for enzyme used is acetate buffer while ethanol is applied as solvent in the enzymatic extraction of stevioside by performing various parameters such as concentration of enzyme, incubation time and temperature. Enzymatic extraction method gives the highest concentration of stevioside (900μg/ml) at 50°C as maximum temperature. The concentration of cellulase at 2% (w/v) gives the highest concentration of stevioside (830μg/ml) and the incubation times of 60 minutes gives the maximum time required to complete the extraction process of stevioside.Therefore, it can be concluded that the extraction of stevioside from Stevia rebaudiana leaves using cellulase is a new efficient way of obtaining high concentration of stevioside and also can minimize the use of solvent and energy consumption in degrading the cell wall. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Stevia rebaudiana |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzymes |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | QK896 .N37 2013 rs Bc. | 0000074637 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 7018 | QK896 .N37 2013 rs Bc. | 0000074638 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |