MARC details
| 000 -LEADER |
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| fixed length control field |
02203nam a2200253 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000076033 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204553.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
131113t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002259(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905241128 |
| Level of effort used to assign nonsubject heading access points |
shamsul |
| Level of effort used to assign subject headings |
201311130846 |
| Level of effort used to assign classification |
nabilah |
| -- |
201311130844 |
| -- |
nabilah |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QP609.P3 Y86 2013 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Muhammad Yunus Zainal Abidin |
| 245 10 - TITLE STATEMENT |
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| Title |
Stability of papain in aqueous organic solvent by reverse phase liquid chromatography / |
| Statement of responsibility, etc. |
Muhammad Yunus Zainal Abidin |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2013 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiii, 49 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 37-38 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Papain is one of the proteolytic enzymes found in the latex of the leaves and of the green fruit of the papaya tree (Carica papaya L.). It’s known to be highly used in the several industry like food, medical, brewing and many more. Hence, the objective of this research is to study the stability of papain in aqueous organic solvent and secondly to purify the papain using the AmberliteTM XAD7HP as the reverse phase liquid chromatography adsorbent. The research is based on experimental lab research. The effects of different concentrations of the water-miscible organic solvents acetonitrile and ethanol on the stability of papain in aqueous solution were studied. Batch adsorption using AmberliteTM XAD7HP was carried out to purify papain from papaya juice. The purified papain was analysed using the Bradford assay, the proteolytic papain activity assay and SDS PAGE. As for conclusion the of the study. The result obtained proved that papain has exhibited higher stability in aqueous acetonitrile than in ethanol under small margin. Decreases in the activity of the enzyme were observed at organic solvent concentrations above 60%. Papain with high purity was achieved by adsorption using the AmberliteTM XAD7HP. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Papain |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Liquid chromatography |