Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis / (Record no. 4319)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02404nam a2200253 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000076039 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251114204554.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 131113t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0004521(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905131527 |
| Level of effort used to assign nonsubject heading access points | azhar |
| -- | 201311130906 |
| -- | nabilah |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | TP248.65.P76 A35 2013 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmad Afifi Ibrahim |
| 245 10 - TITLE STATEMENT | |
| Title | Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis / |
| Statement of responsibility, etc. | Ahmad Afifi Ibrahim |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2013 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xvi, 68 p. : |
| Other physical details | ill. ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 60-63 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The purpose of this study is to investigate the effect of pH and enzyme loading on the concentration of protein during enzymatic hydrolysis of Collocalia Fuciphaga. C. Fuciphaga is refers to the bird nest from one swiftlet species that is used as the sample known to be highly nutritious. Bovine Serum Albumin (BSA), a standard protein solution was used as the benchmark for the reference of the protein concentration. A research had been proposed in this study to optimize the production of protein from C.Fuciphaga. The effect of changing of the parameters values during the enzymatic hydrolysis was studied and the higher protein concentrations produced were highlighted. The standard protein curve of Bovine Serum Albumin (BSA) is prepared first with several dilutions for protein standard curve. The results from two significant parameters in the experiment was taken and analyzed. The selection of parameters values was taken from the enzymes’ optimum condition where the pH value of Alcalase 2.4L is from pH 7 until pH 10 whiles the temperature is fixed at 50oC. Enzyme loading was varies from 1.5% to 3.0% (v/w). The sample was analyzed using UV-Vis Spectrophotometer to determine the concentration of protein in each sample. The optimum condition is found to be at extraction condition of pH 8.5 and enzyme concentration of 3.0% (v/w). Characterization of the sample had been done and validated the protein composition on the extracted sample. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Proteins |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Hydrolysis |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | TP248.65.P76 A35 2013 rs Bc. | 0000074733 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 7066 | TP248.65.P76 A35 2013 rs Bc. | 0000074734 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |