MARC details
| 000 -LEADER |
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| fixed length control field |
02387nam a2200265 a 4500 |
| 001 - CONTROL NUMBER |
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vtls000076046 |
| 003 - CONTROL NUMBER IDENTIFIER |
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KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251114204554.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
131113t2013 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0002134(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131654 |
| Level of effort used to assign nonsubject heading access points |
yusri |
| -- |
201311130921 |
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nabilah |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QD321 .I99 2013 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nurul Izzah Ahmad |
| 245 10 - TITLE STATEMENT |
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| Title |
Characterization and thermostability study of invertase by Aspergillus niger in submerged culture / |
| Statement of responsibility, etc. |
Nurul Izzah Ahmad |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2013 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiv, 41 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 36-38 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Invertase is a commercially important enzyme used for hydrolysis of sucrose. The hydrolysis of sucrose yields a mixture of glucose and fructose, or famous as name invert syrup, this enzyme was widely used in food and beverage industries. Objectives of this research are to study the pH and thermostability of invertase by Aspergillus niger, to study the enzyme kinetics of invertase and to study the effect of sucrose concentration during incubation towards invertase activity. A. niger produced high levels of invertase under culture conditions (potato dextrose agar) on fourth day of incubation at an optimum temperature 30OC, inoculum at 30OC at 250 rpm and using sucrose as a substrate by submerged fermentation (SmF) also with same culture conditions. Separation between enzyme and other cell was done by centrifugation and continued with study the pH and thermostability of enzyme by terminated by heating. Thermostability of enzyme were investigated by determining the enzymatic reaction subjected to 40OC, 45OC, 50OC, 55OC and 60OC. Michaelis-Menten parameters (Vmax and Km) were determined for four different sucrose concentration which are 0.2, 0.4, 0.6, 0.8 g/L. Result suggested that optimum pH is 5.0 and temperature is 55OC. While both Vmax and Km would increase at higher temperatures because temperature will alter the shape of enzyme by changing its ionic form at active site. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Invertase |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Enzymes |
| General subdivision |
Thermal properties |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Aspergillus niger |