Electrophoretic purification of recombinant green fluorescent protein from intact escherichia coli cells in continuous buffer system / (Record no. 5134)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02452nam a2200265 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000084648 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251117113230.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 141223t2014 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | THE0002251(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201905241120 |
| Level of effort used to assign nonsubject heading access points | shamsul |
| -- | 201412231606 |
| -- | fawwaz |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | QP609.C39 C43 2014 r Bc. |
| 100 3# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Chau, King Hou |
| 245 10 - TITLE STATEMENT | |
| Title | Electrophoretic purification of recombinant green fluorescent protein from intact escherichia coli cells in continuous buffer system / |
| Statement of responsibility, etc. | Chau King Hou |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Kuantan, Pahang : |
| Name of publisher, distributor, etc. | UMP, |
| Date of publication, distribution, etc. | 2014 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xvi, 39 p. : |
| Other physical details | ill. ; |
| Dimensions | 30 cm. + |
| Accompanying material | 1 CD-ROM |
| 500 ## - GENERAL NOTE | |
| General note | Faculty of Chemical & Natural Resources Engineering |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2014 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography : p. 27-30 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Green fluorescent protein (GFP) is a protein that consists of 27 kDa protein of 238 amino acid residues. GFP emits bright green fluorescence light when exposed to blue or ultraviolet light. GFP has been used as a marker for the gene expression visualization, protein localization in living and fixed tissues as well as for protein targeting in intact cells and organisms. A direct purification method was developed to purify the recombinant GFP from intact Escherichia coli (E. coli) cells using preparative native polyacrylamide gel electrophoresis (n-PAGE) in continuous buffer system. 100 μL of 12% (w/v) polyacrylamide gel was used to study the effect of biomass concentration and the effect of resolving gel height on the preparative n-PAGE. The amount of purified GFP was determined by using the gel-based imaging method and the Lowry protein determination method to determine the purity and yield of the recovered GFP. The optimal biomass concentration in the feedstock was found at 15% (w/v) with 62.5% of purity. The purity of GFP slightly reduced when the biomass concentration increased to 25% (w/v). Meanwhile, 89% of purity was achieved when 1 cm of resolving gel was employed in preparative n-PAGE. The purity of the GFP decreased when the gel height increased to 2.5cm. However, the percentage of the yield in this study was unable to determine since the calculation was completely offset |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Green fluorescent protein |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Escherichia coli |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | QP609.C39 C43 2014 r Bc. | 0000091683 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report | |||
| Not lost | Not for loan | UMPLIB GAMBANG | UMPLIB GAMBANG | 04/09/2019 | CD 8608 | QP609.C39 C43 2014 r Bc. | 0000091684 | 04/09/2019 | 1 | 04/09/2019 | Final Year Report |