MARC details
| 000 -LEADER |
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| fixed length control field |
02859ntm a2200241 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000083342 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113245.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
141112t2013 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004078(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905160915 |
| Level of effort used to assign nonsubject heading access points |
fauzi |
| Level of effort used to assign subject headings |
201411261255 |
| Level of effort used to assign classification |
fawwaz |
| Level of effort used to assign subject headings |
201411141252 |
| Level of effort used to assign classification |
fawwaz |
| Level of effort used to assign subject headings |
201411121242 |
| Level of effort used to assign classification |
fawwaz |
| -- |
201411121239 |
| -- |
fawwaz |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP156.E8 S27 2013 r Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Siti Sarah Abdullah |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of enzymatic extraction of stevioside from stevia rebaudiana leaves by using cellulase / |
| Statement of responsibility, etc. |
Siti Sarah Abdullah |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2013 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiv, 53 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2013 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 45-48 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Stevioside is one of the components known as diterpene glycoside existing in Stevia rebaudiana leaves which have 250 to 300 sweeter than sucrose at the concentration of 0.4% (w/v). It is used as sweetening agents and taste modifier mostly in food industry. Moreover, stevioside has no calorific value and suitable used in therapeutic especially in treating diabetic people. The objective of this research is to optimize the extraction of stevioside from Stevia rebaudiana leaves by using the cellulase. A Central Composite Design (CCD) has been chosen to study the effect of enzyme concentration, incubation time and temperature on extraction of stevioside. The medium for enzyme used is acetate buffer while ethanol is applied as solvent in the enzymatic extraction of stevioside by performing various parameters such as concentration of enzyme, incubation time and temperature. The p-value for cellulase concentration and incubation time was less than 0.05 indicate that these factors have significant effects on the extraction yield of stevioside. The extraction of stevioside is represented by a quadratic model with p-value <0.0001. From the experimental work, an enzymatic extraction method gives the highest concentration of stevioside (840.839 μg/ml) at 45°C as maximum temperature, concentration of cellulase at 1.75% (w/v) and the incubation times of 105 minutes gives the maximum time required to complete the extraction process of stevioside. The extraction stevioside yield in this experiment gets more than predicted values (835.49μg/ml). However the experimental value is not much difference compare to the predicted value. The extraction of stevioside from Stevia rebaudiana leaves using cellulase is a new efficient way of obtaining high concentration of stevioside and can minimize the use of solvent and energy consumption in degrading the cell wall |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Extraction (Chemistry) |