MARC details
| 000 -LEADER |
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| fixed length control field |
03886ntm a2200265 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000087845 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113257.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150605t2014 my a f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004580(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905170919 |
| Level of effort used to assign nonsubject heading access points |
azhar |
| Level of effort used to assign subject headings |
201711281351 |
| Level of effort used to assign classification |
saini |
| Level of effort used to assign subject headings |
201506121555 |
| Level of effort used to assign classification |
ariffin |
| Level of effort used to assign subject headings |
201506121125 |
| Level of effort used to assign classification |
ariffin |
| -- |
201506051009 |
| -- |
ariffin |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP358 .N66 2014 r Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Noordiana Ariffin |
| 245 10 - TITLE STATEMENT |
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| Title |
Production of ethanol by immobilized saccharomyces cerevisiae / |
| Statement of responsibility, etc. |
Noordiana Ariffin |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2014 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xv, 58 p. : |
| Other physical details |
ill. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Chemical and Natural Resources Engineering |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang -- 2014 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
This research was conducted on the fermentation of ethanol by Saccharomyces cerevisiae using immobilization technique. S. cerevisiae which is also known as Baker’s yeast is a single-celled eukaryote that is frequently used in scientific research. It is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated and very easy to maintain in the laboratory. Besides, immobilization has already been approved as useful method in maintaining high cell concentration within the reactor, increases the cell’s ability to tolerate high ethanol concentration in the medium, allowing higher conversion yield and increased volumetric productivities. Therefore, the purpose of this study is to immobilize the yeast using various kind of supports (e.g: muslin cloth, membrane and sugarcane stalk) in batch processes to determine the best support for ethanol production. In this method, immobilized fermentation was carried out through shake flask seeding technique while the duration of fermentation process was fixed at 10 hours with sampling made at every 2 hours intervals. The germination of the stock and seed culture preparations were conducted followed by the immobilization technique in order to produce ethanol. Samples taken were analyzed for ethanol and glucose concentration, colony forming unit (CFU), biomass concentration, optical density and viewing of S. cerevisiae attachment on immobilized supports by Scanning Electron Microscope (SEM). From the result obtained, it showed that immobilized cloth was capable to increase cell optical density up to 1.602 after 10 hours of fermentation. Moreover, analysis performed by using biomass has revealed that immobilized cloth at 10 hours of fermentation gave the highest biomass concentration which is 39.64 gL-1 and colonies of cells forming at 2.57 x 108/mL of viable cells compared to other materials. Besides, its ethanol productivity was enhanced to 0.42 gL-1h-1, being over 9.6% higher than that observed in the batch culture (0.38 gL-1h-1). The consumption of glucose was improved by 6.9% (compared to batch operation), where nearly 67% of glucose conversion at 10 hours period of immobilized fermentation through muslin cloth. The increment might be associated with the altered metabolical functions in the immobilized cells. This alteration is attributed to the reduction of the diffusion path of the growth nutrient that enhanced the availability and promoted the growth of yeast, thus improving the catalytic conversion of glucose to ethanol. Therefore, the supported muslin cloth was proved to increase the number of cells and glucose inhibition is able to reduce with the utilization of immobilized S. cerevisiae, thus increasing ethanol production |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Alcohol as fuel |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://ecollib.ump.edu.my/id/eprint/9490">http://ecollib.ump.edu.my/id/eprint/9490</a> |
| Public note |
Access in library only |