MARC details
| 000 -LEADER |
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| fixed length control field |
02267nam a2200241 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000058721 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113258.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
120319t2010 my da f m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0004134(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905132119 |
| Level of effort used to assign nonsubject heading access points |
farhana |
| Level of effort used to assign subject headings |
201203191122 |
| Level of effort used to assign classification |
Fida |
| -- |
201203191120 |
| -- |
Fida |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
TP156.F4 N677 2010 rs Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Norsalita Abd Talib |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of pullulanase activity through design of experiment (DOE) / |
| Statement of responsibility, etc. |
Norsalita Abd Talib |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2010 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiii, 55 p. ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2010 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Enzyme has been used widely in industries today due to its capability in catalyzing various types of biochemical reactions. However, the high cost of enzyme gives problems to the enzyme industries. A research was done to optimize the pullulanase activity from Bacillus Acidopullulyticus using experimental design. Two level Full Factorial design was applied in the screening process of four parameters for pullulanase activity and only three parameters were recognized as significant and effective factors for pullulanase activity. The three significant parameters were pH, enzyme and substrate concentration. Thus the three parameters were selected for further optimization studies using response surface methodology (RSM) in Design Expert version 7.1.6 software with Central Composite Design (CCD) mode. Thirty four sets of experiments with different parameter values were suggested. The predicted optimum values with R2 of 0.8729 for pH, enzyme concentration, substrate concentration and pullulanase activity were 6.60, 11.11 U/ml, 4.44% (w/v) and 3.17 U/ml respectively. One validation experiment was run using the optimized parameter and resulted 3.05 U/ml pullulanase activities as compared to the suggested values of 3.17 U/ml. As a conclusion, this research was successful to increase the pullulanase activity with a minimum numbers of experiments. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Pullulanase |