MARC details
| 000 -LEADER |
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| fixed length control field |
03751ntm a2200289 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000094233 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113318.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
160404t2015 da f ab m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0000181(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131652 |
| Level of effort used to assign nonsubject heading access points |
nazirah |
| Level of effort used to assign subject headings |
201710120954 |
| Level of effort used to assign classification |
aishah |
| Level of effort used to assign subject headings |
201604041109 |
| Level of effort used to assign classification |
shima |
| -- |
201604041029 |
| -- |
shima |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .N66 2014 r Thesis |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Noorhidayah Md Nazir |
| 245 1# - TITLE STATEMENT |
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| Title |
Identification of unique DNA sequences in Aquilaria malaccensis and Aquilaria hirta via genomic representational difference anaylsis (RDA)/ |
| Statement of responsibility, etc. |
Noorhidayah Md Nazir |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2014 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xv, 116 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Science and Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Master of Science (Biotechnology))-- Universiti Malaysia Pahang – 2014 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 92-102 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Aquilaria species that are listed among the endangered plant species are well known as the main agarwood producers in the world. Agarwood produced by Aquilaria spp. has been widely used in traditional medicine, incenses for religious rituals and also in perfumery industry. However, identification of the species in Aquilaria genus is very challenging due the changing of their morphological structures that are highly dependent on the changes of environmental conditions. Thus, this study was aimed to identify the unique DNA sequences that are available in the genome of Aquilaria malaccensis and A. hirta to be used as specific molecular markers for species identification purpose. Genomic Representational Difference Analysis (RDA) was applied by amplifying DNA sequences that are only available in the genome of interest species while eliminating DNA sequences that are redundant in both interest and noninterest genomes. A number of three rounds of RDA with different ratios of “tester” and “driver” DNA were carried out and finally, 15 unique DNA sequences were successfully isolated from the final round of RDA. The isolated sequences were sequenced and subjected to BLASTN analysis for identification and prediction of their functional role based on their similarity to homologous DNA sequences that are available in the NCBI GenBank database. From the BLASTN analysis, majority of the sequences did not match to any sequence that is available in the database. This might be due to the lacking of information discovered and reported on Aquilaria spp. in molecular biology as well as bioinformatics aspects. Based on the isolated sequences, different set of gene specific primers were designed specifically for each potential sequence. The primers were subsequently applied in PCR amplification of the unique DNA sequences directly from genomic DNA of selected Aquilaria spp. for confirmation of the presence of the DNA sequences uniquely in species of interest while absence in the other Aquilaria spp.. From the confirmation, 12 unique DNA sequences have shown their potential to be used as molecular markers in A. hirta while in A.malaccensis, there are only three identified potential sequences to be used as molecular markers. The isolated unique DNA sequences will be very useful for Aquilaria spp. identification that will benefit agarwood plantation industry in order to produce and maintain a good quality of agarwood for fragrance and perfumery industries. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Science and Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Universities and Colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Theses |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://ecollib.ump.edu.my/3665/">http://ecollib.ump.edu.my/3665/</a> |
| Public note |
Library access only |