MARC details
| 000 -LEADER |
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| fixed length control field |
04013ntm a2200289 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000097793 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113336.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
160919t2016 my da f abm 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0000108(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905131144 |
| Level of effort used to assign nonsubject heading access points |
nazirah |
| Level of effort used to assign subject headings |
201710090958 |
| Level of effort used to assign classification |
aishah |
| -- |
201609191507 |
| -- |
saini |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .A36 2016 r Thesis |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mohd Aiman Barudin |
| 245 10 - TITLE STATEMENT |
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| Title |
Molecular characterization of fragrance-related transcripts in benzenoid/phenylpropanoid pathway of Vanda Mimi Palmer / |
| Statement of responsibility, etc. |
Mohd Aiman Barudin |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2016 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xxx, 219 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Sciences and Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Master of Science in Biotechnology (by Research)) -- Universiti Malaysia Pahang – 2016 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 167-183 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Vanda Mimi Palmer (VMP) is a scented orchid that has received several international recognitions for its strong-sweet fragrance containing high percentage of benzenoids as well as phenylpropanoid compounds. Unfortunately, knowledge on the biosynthesis of benzenoids and phenylpropanoid compounds is still not fully-understood especially in Vandaceous orchid. Thus, this study was aimed to identify, isolate and molecular characterise potential transcripts that might be involved in the biosynthesis of benzenoids and phenylpropanoids in VMP. Screening of fragrance-related transcripts via cDNA-RDA approach has shown identification of partial sequences of phenylalanine ammonia lyase (VMPPAL) and S-adenosyl-L-methionine synthase (VMPSAMS) from VMP besides methionine synthases from Vanda Small Boy Leong (VSBLMS) and Vandachostylis Sri-Siam (VSSSMS). However, those transcripts were not further characterised due to their indirect involvement in final fragrance compounds biosynthesis. Thus, previously identified partial transcripts from floral cDNA library of VMP were isolated including 3-keto-acyl CoA thiolase (VMPKAT), benzoic acid/salicylic acid carboxyl methyltransferase (VMPBSMT), eugenol synthase (VMPEGS) and orcinol-O-methyltransferase (VMPOOMT), which resulted in the isolation of full ORF encoding 450, 378, 306 and 368 amino acids, respectively. All the transcripts were further characterised using molecular and bioinformatics approaches including domains identification, comparative modeling, molecular docking for substrates and ligands prediction as well as gene expression analysis using real-time RT-PCR. In this study, 3D-structural model was developed on those fragrance-related proteins for comparative modeling and molecular docking. Molecular docking on those proteins has shown their suitability towards specific substrates and ligands, reflecting their homologous putative functionality to fragrance-related proteins from other well-studied scented plants. However, gene expression analysis using real-time RT-PCR has shown that VMPKAT and VMPBSMT might be involved in benzenoids biosynthesis due to their up-regulated expression in floral tissues as well as developmentally-regulated expression pattern. Meanwhile for VMPEGS and VMPOOMT genes, their gene expression pattern might suggest their involvement as intermediates for biosynthesis of vanillin or other non-fragrance benzenoid and phenylpropanoid compounds, respectively. In conclusion, VMPKAT and VMPBSMT might be involved in fragrance benzenoid biosynthesis based on their functional catalytic prediction, up-regulated gene expression in floral compared to vegetative tissues and their developmentally-regulated expression pattern with the highest transcripts in fully-open flower. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Sciences and Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and Colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Theses |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://ecollib.ump.edu.my/3509/">http://ecollib.ump.edu.my/3509/</a> |
| Public note |
Library access only |