MARC details
| 000 -LEADER |
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| fixed length control field |
03072ntm a2200277 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000101453 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113358.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
171002s2017 my a f am 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
101453 |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905141007 |
| Level of effort used to assign nonsubject heading access points |
nazirah |
| Level of effort used to assign subject headings |
201710021610 |
| Level of effort used to assign classification |
saini |
| -- |
201710021603 |
| -- |
saini |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .R69 2017 r Thesis |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Roziasyahira Mutazah |
| 245 10 - TITLE STATEMENT |
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| Title |
Chemical constituents, antioxidant and cytotoxicity properties of Rhodomyrtus Tomentosa root extracts / |
| Statement of responsibility, etc. |
Roziasyahira Mutazah |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2017 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiv, 107 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Sciences and Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Master of Science) -- Universiti Malaysia Pahang – 2017 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Bibliography : p. 66-78 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A study was carried out on the chemical constituents, antioxidant and cytotoxicity properties of Rhodomyrtus tomentosa root extracts. Solvent–solvent extraction, fractionation and separation using different chromatographic techniques (column chromatography and thin layer chromatography) were used for isolation of pure compounds. The chemical constituents of the roots of R. tomentosa were subjected to ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-QToF/MS) for the determination of compound composition present in the plant. The potential cytotoxicity of R. tomentosa extracts from different solvents were evaluated in vitro on HepG2, MCF-7 and HT 29 cell lines and antioxidant activity was monitored by radical scavenging assay (DPPH), copper reducing antioxidant capacity (CUPRAC) and β-carotene bleaching assay. Each extract from R. tomentosa inhibited proliferation on all cancer cell lines in a concentration-dependant manner. According to the IC50 obtained, the results exhibited significant activity against cancer cell line under 72h incubation with IC50 value < 30 μg/mL for ethyl acetate on HepG2, methanol and ethyl acetate on both MCF-7 and HT 29. Methanol extracts show significant antioxidant activities in DPPH, CUPRAC and β-carotene due to the presence of high total flavonoid and total phenolic content of R. tomentosa. Bioassay guided fractionation of ethyl acetate extract led to the isolation of lupeol and fractionation of chloroform extract led to the isolation of β-sitosterol. The structure of the isolated compounds were elucidated by spectroscopic methods such as 1D (1H, 13C, DEPTQ), 2D (COSY, HMQC, HMBC) NMR, MS, UV, FTIR and also by comparison with the literature. Collectively, the results show that R. tomentosa is a potential source of antioxidant and cytotoxicity and identified lupeol being a possible promising chemical lead for further development as anticancer agent. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Sciences and Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Universities and Colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Theses |