MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03607ntm a2200373 i 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000102809 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113359.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
180118s2017 my da f a m 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0000981(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
|---|
| Level of rules in bibliographic description |
201905241626 |
| Level of effort used to assign nonsubject heading access points |
nazirah |
| Level of effort used to assign subject headings |
201802081544 |
| Level of effort used to assign classification |
fateeha |
| Level of effort used to assign subject headings |
201802021152 |
| Level of effort used to assign classification |
fateeha |
| Level of effort used to assign subject headings |
201801231143 |
| Level of effort used to assign classification |
fateeha |
| -- |
201801181553 |
| -- |
fateeha |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
UMP |
| Language of cataloging |
eng |
| Transcribing agency |
UMP |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FKKSA .N87 2017 r Bc. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nursyahira Mohd Rafi, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Effect of freeze and thaw cycle and incubation period on the solubilisation of inclusion body protein / |
| Statement of responsibility, etc. |
Nursyahira Mohd Rafi |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Kuantan, Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMP, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2017 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
© 2017 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xiii, 70 pages : |
| Other physical details |
illustrations (some color), charts ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Source |
rdacontent |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
unmediated |
| Source |
rdamedia |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
computer |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Source |
rdacarrier |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
computer disc |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
|---|
| File type |
text file |
| Encoding format |
PDF |
| Source |
rda |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Faculty of Chemical & Natural Resources Engineering |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Project Paper (Bachelors of Chemical Engineering) -- Universiti Malaysia Pahang – 2017 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Overexpression of recombinant protein in bacteria result in the formation of inactive protein. These inactive proteins associate forming insoluble protein aggregates which is referring to inclusion bodies (IBs). Generally, IBs are pure and the aggregated protein inside it has native-like secondary structure which is a bioactive protein. To recover the insoluble and active protein is a major problem encountered. Solubilisation does play a crucial role by unfolded the protein and thus help it to refold properly so that functional bioactive protein can be recovered. Example of mild solubilisation method using low concentration of urea and combine with freeze and thaw method has been proven to increase the efficiency of the recovering of bioactive protein. For freeze and thaw process there are factors that affect the overall process which are freezing incubation period and number of process cycle. Incubation period affect the process by determining the amount of stress needed to be applied so that unfolding process can occur. Number of cycle does affect the protein stability in terms of the occurrence of protein degradation probability. Thus the objective of this research is to investigate effect of freeze and thaw cycle an incubation period on the solubilisation of IBs. For incubation period the experiment was conducted between 1 to 4 days whereas for freeze and thaw cycle experiment conducted between cycle 1 to 4. Moreover, the performance of these two parameters were analysed using native-polyacrylamide gel electrophoresis (n-PAGE) to determine the functional for enhance green fluorescent protein (EGFP) amount and Bradford assay to determine the total protein amount. In this study, incubation period did affected the performance of solubilisation rate in which the IBs being solubilised and then proceed for refolding process and has been proven achieved active form of EGFP. For number of process cycle, it did not affect the solubilisation rate on determining the amount of functional EGFP recovered. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Chemical & Natural Resources Engineering |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Universities and Colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Theses |