MARC details
| 000 -LEADER |
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| fixed length control field |
03624ntm a2200277 a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
vtls000101507 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
KUKTEM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251117113359.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
171003t2017 my a f am 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0000927(Local) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
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| Level of rules in bibliographic description |
201905241646 |
| Level of effort used to assign nonsubject heading access points |
nazirah |
| -- |
201710031239 |
| -- |
nazri |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FKKSA .K36 2017 r Thesis |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Kamaliah Abdul Samad |
| 245 10 - TITLE STATEMENT |
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| Title |
Synergistic ferulic acid production from banana stem waste by co-culture / |
| Statement of responsibility, etc. |
Kamaliah Binti Abdul Samad |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Kuantan, Pahang : |
| Name of publisher, distributor, etc. |
UMP, |
| Date of publication, distribution, etc. |
2017 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvii, 202 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Chemical and Natural Resources Engineering |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Doctor of Philosophy (Chemical Engineering)) -- Universiti Malaysia Pahang β 2017 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliography : p. 145-169 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacterial co-culture fermentation technique using banana stem waste (BSW) as substrate was implemented to improve FA production. Soil microbes were isolated and preliminary study was performed to select the best bacterial strain. Subsequently, 26 type of bacterial co-cultures were formed and evaluated their ability dependent on FA production. Optimization of the FA production was conducted by employing response surface methodology using the selected co-culture as inoculum. A kinetic study was carried out to determine kinetic constants using Michaelis-Menten equation. Meanwhile, the enzyme assay was performed to investigate the mechanism release of FA by enzymatic hydrolysis. Ferulic acid production was accomplished through submerged fermentation and the sample was analyzed by HPLC to quantify the FA content. The result found that 5 out of 46 isolated bacterial strains were efficient in releasing FA from BSW. However, the highest production of FA was observed in co-culture A (Bacillus cereus CCM 2010, Bacillus pumilus SAFR-032 and Bacillus thuringiensis Bt407), followed by co-culture B (Bacillus cereus CCM 2010, and Bacillus thuringiensis Bt407). In improving the fermentation process, screening by fractional factorial design was performed and the result obtained presented four factors including pH, rotation rate, type of co-culture, and volume of inoculum have significant effects on FA production. Furthermore, rotation rate at 150 rpm and volume of inoculum of 5 % were found to be optimum in increasing FA yield up to 510.24 mg/kg within 24 h using bacterial co-culture B as an inoculum. Meanwhile, the kinetic constant of ππππ₯, πΎπ and πΎπ were reported as 0.0003 g L-1 h-1, 1.636 g L-1 and 0.0095 h-1, respectively using Runge-Kutta Forth method. Besides, the maximum activity of ferulic acid esterase (0.046 mU/mL) was detected in the stationary phase of co-culture growth. The result proved that the enzymatic hydrolysis was taking place to break down the cell wall of BSW in releasing FA. The finding of this study suggests that co-culture could induce the synergistic work in improving the FA production from banana stem waste during the fermentation process. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Chemical & Natural Resources Engineering |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and Colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Theses |