Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry / (Record no. 7764)

MARC details
000 -LEADER
fixed length control field 03029ntm a2200349 i 4500
001 - CONTROL NUMBER
control field vtls000103684
003 - CONTROL NUMBER IDENTIFIER
control field KUKTEM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20251117113402.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 180531s2018 my a f am 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0001430(Local)
039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE]
Level of rules in bibliographic description 201905211441
Level of effort used to assign nonsubject heading access points asmadi
-- 201805311438
-- saini
040 ## - CATALOGING SOURCE
Original cataloging agency UMP
Language of cataloging eng
Transcribing agency UMP
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FTeK .T363 2018 r Bc.
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Tan, Darlene Tzet Lene,
Relator term author.
245 10 - TITLE STATEMENT
Title Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry /
Statement of responsibility, etc. Darlene Tan Tzet Lene
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Kuantan, Pahang :
Name of producer, publisher, distributor, manufacturer UMP,
Date of production, publication, distribution, manufacture, or copyright notice 2018
300 ## - PHYSICAL DESCRIPTION
Extent xiii, 71 pages :
Other physical details illustrations (some color) ;
Dimensions 30 cm. +
Accompanying material 1 CD-ROM
336 ## - CONTENT TYPE
Content type term text
Source rdacontent
337 ## - MEDIA TYPE
Media type term unmediated
Source rdamedia
337 ## - MEDIA TYPE
Media type term computer
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term volume
Source rdacarrier
338 ## - CARRIER TYPE
Carrier type term computer disc
Source rdacarrier
347 ## - DIGITAL FILE CHARACTERISTICS
File type text file
Encoding format PDF
Source rda
500 ## - GENERAL NOTE
General note Faculty of Engineering Technology
502 ## - DISSERTATION NOTE
Dissertation note Project Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2018
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references
520 3# - SUMMARY, ETC.
Summary, etc. The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at 𝑅𝑓 value of 0.22. However, in HPLC analysis, the caffeic acid was not detected in sample at the retention time of 3.82 minutes as per reference of the standard caffeic acid chromatogram. Split peak and baseline drift were also observed in WEP during HPLC analysis and requires troubleshooting process. WEP showed lower amount of total flavone, flavonols and total phenolic contents as compared to ethanolic extracted peopolis (EEP) and olive oil extracted propolis (OEP). For TLC, chrysin components was detected in EEP whereas none of the components were detected by OEP. For HPLC, caffeic acid were not detected all three propolis extracts.
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Engineering Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Disertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Theses
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not lost   Not for loan UMPLIB GAMBANG UMPLIB GAMBANG 04/09/2019   CD 11344 | FTeK .T363 2018 r Bc. 0000122923 04/09/2019 1 04/09/2019 Final Year Report
  Not lost   Not for loan UMPLIB GAMBANG UMPLIB GAMBANG 04/09/2019   FTeK .T363 2018 r Bc. 0000122922 04/09/2019 1 04/09/2019 Final Year Report

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