MARC details
| 000 -LEADER |
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| fixed length control field |
04082nam a2200361 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20251125105756.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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a||||fr|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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201105t20202020my a|||frma|| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0008567(Local) |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| Language of cataloging |
eng |
| Transcribing agency |
UMP |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .P36 2020 r Thesis |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Pang Wei Cheng, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Isolation, gene expression and structural analysis of fruit bromelain from ananas comosus cultivar MD 2 / |
| Statement of responsibility, etc. |
Pang Wei Cheng |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Kuantan, Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMP , |
| Date of production, publication, distribution, manufacture, or copyright notice |
2020 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
©2020 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvii, 146 pages : |
| Other physical details |
illustrations (some color) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD ROM |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Source |
rdacontent |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
unmediated |
| Source |
rdamedia |
| 337 ## - MEDIA TYPE |
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| Media type term |
computer |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
volume |
| Source |
rdacarrier |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
computer disc |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| File type |
text file |
| Encoding format |
PDF |
| Source |
rda |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Sciences & Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Master of Science ) -- Universiti Malaysia Pahang – 2020 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Ananas comosus, or commonly known as pineapple, is one of the most popular tropical fruits in the world. There are various pineapples cultivars with distinct characteristics. A. comosus cultivar MD 2 is the most successful pineapple hybrid with its attractive gold colour, super sweet taste and superior quality. Pineapple is rich in proteolytic enzymes such as fruit bromelain. In this study, fruit bromelain was characterised and compared between ripe and unripe A. comosus cultivar MD 2. Besides that, fruit bromelain transcripts were also isolated by conventional PCR and cloning. The extracted fruit bromelain sequence information was used in tertiary structure modelling followed by thermostability analysis. To investigate the gene expression level and proteolytic activity of fruit bromelain of A. comosus cultivar MD 2, quantitative Polymerase Chain Reaction (PCR) and casein enzymatic assay were conducted. Meanwhile, the sequence information of fruit bromelain was analysed using several bioinformatic tools including BioEdit, BLAST, InterProScan and ProtParam. The tertiary structure of the selected fruit bromelain sequences was modelled using MODELLER and refined via molecular dynamics simulation. Subsequently, the thermostability of fruit bromelain was evaluated using casein enzymatic assay and molecular dynamics simulation. The result revealed that fruit bromelain was down-regulated by 90 % in ripe A. comosus cultivar MD 2. It was also found that unripe A. comosus cultivar MD 2 has higher fruit bromelain activity (1.91 ± 0.08 U/mL) than ripe A. comosus cultivar MD 2 (1.13 ± 0.09 U/mL). On the other hand, three fruit bromelain sequences namely OAY62650.1, OAY68270.1 and OAY85858.1 were selected as sequences of interest to be isolated and cloned. The sequencing result demonstrated several nucleotides alteration in the isolated sequences. Moreover, Verify 3D, ERRAT and PROCHECK showed that the generated fruit bromelain models have high stereochemical quality. Structural analysis revealed interactions between the pro-domain and catalytic domain. Binding subsites of fruit bromelain His160, S2 Trp183, Glu19 and Asn159 were also identified. The His160, Trp183 and Glu19 were found conserved between fruit bromelain and papain. In addition, the thermal profile of MD 2 fruit bromelain was constructed. The optimum temperature of fruit bromelain was identified at 60 °C which is within the expected range. As the temperature raises, the stability of fruit bromelain was disrupted. The simulation result showed that fruit bromelain structures destruction may be due to flexible secondary loops as well as the presence of non-charged and hydrophilic amino acids residues. The information obtained from this study is expected to improve the applications of fruit bromelain in the future. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Sciences & Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Dissertations |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |