MARC details
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04735ntm a2200385 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20260929121816.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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t||||fr|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
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201125t20142014my a|||fr|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0009014(Local) |
| Qualifying information |
hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| Language of cataloging |
eng |
| Transcribing agency |
UMP |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FIST .S58 2014 r Thesis |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Shuhada Abdul Muttalib, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Bioconversion of isoeugenol to vanillin with different strains of pseudomonas aeruginosa / |
| Statement of responsibility, etc. |
Shuhada Abdul Muttalib |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Kuantan, Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMP, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2014 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Date of production, publication, distribution, manufacture, or copyright notice |
©2014 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvii, 85 pages : |
| Other physical details |
illustrations (some color) ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Source |
rdacontent |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
unmediated |
| Source |
rdamedia |
| 337 ## - MEDIA TYPE |
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| Media type term |
computer |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
volume |
| Source |
rdacarrier |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
computer disc |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| File type |
text file |
| Encoding format |
PDF |
| Source |
rda |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Industrial Sciences and Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Master of Science in Biotechnology) -- Universiti Malaysia Pahang – 2014 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The experiment was conducted at the Faculty of Industrial Sciences and Technology lab at Universiti Malaysia Pahang to investigate the bioconversion of isoeugenol to vanillin. Vanillin is a simple monoterpenoid which is considered as one of the world’s principal flavouring compound used extensively in the food, beverage, perfumery, and pharmaceutical industries. Vanillin can be produced using bioconversion of isoeugenol via microorganism and it could be used to substitute synthetic vanillin with a natural vanillin flavor at an affordable price. This study was conducted to screen the Pseudomonas aeruginosa strains for the bioconversion of isoeugenol to vanillin. Initially isoeugenol was obtained from extraction of crude clove bud oil. Two different methods of extraction were done to extract the crude clove bud oil which were microwave extraction and steam distillation. Through microwave extraction of clove bud oil, eugenol can be extracted at minimum time of 75 minutes with an optimum yield of 9.09% as compared to the steam distillation technique where it took time to achieve higher yield of eugenol. Purified eugenol (purity ≥99%) was obtained using 1.2 moles of sodium hydroxide with recycle water. Ruthenium acetylacetonate was used as catalyst to produce isoeugenol by synthesis. The conversion was almost 99% but the method is very expensive and cannot be further use as a substrate in biotransformation process. API-20E test was selected as a biochemical test to identify the characteristics of Pseudomonas aeruginosa strains P178, U641, S376, B932 and ETT187. In fact, all Pseudomonas aeruginosa strains were also confirmed using 16S rRNA gene sequencing and obtained that all the strains were Pseudomonas aeruginosa. In this study, the subculture of different strains of Pseudomonas aeruginosa was used to convert isoeugenol to vanillin by oxidation. Vanillin formation was analyzed directly by gas chromatography mass spectrometry (GCMS). All the strains exhibited good potential as whole-cell bio-catalysts for direct bioconversion of isoeugenol to vanillin. During biotransformation screening by whole cell culture of P. aeruginosa strains, P. aeruginosa ETT187 showing a good vanillin produced which is 2.312±0.006 g/l at only 1% (v/v) isoeugenol added for 24 hours incubation at 200 rpm agitation. Furthermore, the effect of vanillin production versus time with 1% induction of isoeugenol was observed at 12, 24, 36, 48, 60, 72, 84, and 96 hours. P. aeruginosa P178 demonstrated consist the highest production of vanillin which was 2.97g/l at 72 hours of incubation while the isoeugenol decreased over time. Meanwhile, P. aeruginosa ETT 187 presented the highest amount of vanillin produced in only 24 hours with 2.31 g/l. Furthermore, strains U641, S376 and B932 produced the highest amount of vanillin at maximum of 96 hours with 2.62 g/l, 3.56 g/l and 2.49 g/l respectively. The reaction also produced the following by-products, namely, isovanillic acid and isovanillin, ethyl vanillate and also vanillyl methyl ketone. As a conclusion, the P. aeruginosa strains which were P. aeruginosa P178, P. aeruginosa U641, P. aeruginosa S376, P. aeruginosa B932 and P. aeruginosa ETT187 can be proposed to pilot scale as bicatalytic to convert isoeugenol to vanillin at a reasonable price. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Industrial Sciences and Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Theses |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |