Cultivation of microalgae spirulina sp. In palm oil mill effluent for essential fatty acids production / (Record no. 96375)

MARC details
000 -LEADER
fixed length control field 04118ntm a2200361 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field MY-KuUP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20251125105950.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field t||||fr|||| 000 0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 220411s2021 my a|||frm||| 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0009201(Local)
Qualifying information hardback
040 ## - CATALOGING SOURCE
Original cataloging agency UMP
Language of cataloging eng
Transcribing agency UMP
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FIST .K37 2021 r Thesis
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Karthick Murugan Palanisamy,
Relator term author.
245 10 - TITLE STATEMENT
Title Cultivation of microalgae spirulina sp. In palm oil mill effluent for essential fatty acids production /
Statement of responsibility, etc. Karthick Murugan Palanisamy
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Kuantan, Pahang :
Name of producer, publisher, distributor, manufacturer UMP,
Date of production, publication, distribution, manufacture, or copyright notice 2021
264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice © 2021
300 ## - PHYSICAL DESCRIPTION
Extent xv, 178 pages :
Other physical details illustrations ;
Dimensions 30 cm. +
Accompanying material 1 CD-ROM
336 ## - CONTENT TYPE
Content type term text
Source rdacontent
336 ## - CONTENT TYPE
Content type term text
Source rdacontent
337 ## - MEDIA TYPE
Media type term unmediated
Source rdamedia
337 ## - MEDIA TYPE
Media type term computer
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term volume
Source rdacarrier
338 ## - CARRIER TYPE
Carrier type term computer disc
Source rdacarrier
347 ## - DIGITAL FILE CHARACTERISTICS
File type text file
Encoding format PDF
Source rda
500 ## - GENERAL NOTE
General note Faculty of Industrial Sciences and Technology
502 ## - DISSERTATION NOTE
Dissertation note Thesis (Master of Science ) -- Universiti Malaysia Pahang – 2021
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references
520 3# - SUMMARY, ETC.
Summary, etc. Essential fatty acids providing health benefits and has increased the consumption as dietary supplements. Omega-3 fatty acids, usually derived from fatty fish, are present in animals, transgenic plants, fungi and many microorganisms, bringing excessive pressure on global fish stocks. Fortunately, microalgae are the primary which are rich in essential fatty acid and present a promising source of omega-3 fatty acid. The cost of nutrient media becomes an obstacle for cultivation of microalgae in larger industry. In this study, total 16 indigenous microalgae been identified and six monoculture of different species such as Amphora sp., Anabaena sp., Chlorella sp., Scenedesmus sp., Spirulina sp., and Tetraselmis sp., were isolated in laboratory environment by using serial dilution and streaking plate techniques from the marine sample collected East costal region of Kuantan, Pahang. These species were tested the growth and survivability in mixture of 5% (v/v) POME + conventional medium. Among these, Spirulina sp. shown higher growth and higher survivability while evaluating and chosen for further experiment. The growth of Spirulina sp. was tested by feeding POME as an only medium with different concentration (10-50% v/v) to maximize the biomass production. Followed with different effect of light intensities (1000-4000 lux) and temperature (21-36±2 ℃). The growth rate of Spirulina sp. was measured by biomass concentration (mg/L) and Chlorophyl content (µg/mL). The highest biomass production of Spirulina sp. was found in the optimum conditions of 30% v/v of POME, 3000 lux light intensity and 31±2 ℃ temperature with highest biomass yield 1.54±0.11 g/L after 18 days of cultivation. The biomass of Spirulina sp. was separated from the culture medium by centrifugation at 6000 g and dried at 70±3 ℃. Dried biomass was treated with hexane and extracted the highest lipid from Soxhlet extractor (44.9±6.1%) as compared to ultrasound assisted (32.3±6.5%) and Bligh and Dyer method (12.3±4.0%). The extracted lipids were trans esterified using methanolic KOH (1:1 v/v) for 120 min at 60±2℃ with aim of obtaining fatty acid methyl ester. Fatty acid methyl ester sample added with internal standard heptadecanoic acid which diluted with octane at 15 mg/ml. 1 µL of samples were analyzed using gas chromatography. Among 13 fatty acids found that, oleic acid (70.16%), palmitoleic acid (13.07%), linoleic acid (8.62%), and stearic acid (2.11%) and ℽ-linolenic acid (0.43%) as dominant fatty acids. Spirulina sp. lipid have great potential to be use in nutraceutical and pharmaceutical product due to its composition of essential fatty acids.
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Industrial Sciences and Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Theses
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Thesis
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not lost Library of Congress Classification   Not for loan Reference UMPLIB GAMBANG UMPLIB GAMBANG Reference 13/01/2022   FIST .K37 2021 r Thesis T000001555 30/06/2022 1 13/01/2022 Thesis
  Not lost Library of Congress Classification   Not for loan Reference UMPLIB GAMBANG UMPLIB GAMBANG Reference 11/04/2022   CD12947 T000001556 30/01/2023 1 11/04/2022 Thesis

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