MARC details
| 000 -LEADER |
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| fixed length control field |
03379ntm a2200373 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251125110113.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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t||||fr|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
220914t20222022my a|||fr|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0009430 (Local) |
| Qualifying information |
hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| Language of cataloging |
eng |
| Transcribing agency |
UMP |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
KK .R34 2022 r Thesis |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Rafidah Mohamed Ali Hanapiah, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Production of xylonic acid from xylose using recombinant E. Coli bl21 (DE3): |
| Remainder of title |
effect of medium requirement / |
| Statement of responsibility, etc. |
Rafidah Mohamed Ali Hanapiah |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMP, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2022 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
© 2022 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiii, 72 pages: |
| Other physical details |
Illustration ; |
| Dimensions |
30 cm.+ |
| Accompanying material |
1 CD ROM |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Source |
rdacontent |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
unmediated |
| Source |
rdamedia |
| 337 ## - MEDIA TYPE |
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| Media type term |
computer |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
volume |
| Source |
rdacarrier |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
computer disc |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| File type |
text file |
| Encoding format |
PDF |
| Source |
rda |
| 500 ## - GENERAL NOTE |
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| General note |
College of Engineering |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Master of Science) -- Universiti Malaysia Pahang – 2020 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical reference |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Xylonic acid (XA) is a valuable organic acid that has various applications similar to other sugar acids such as gluconic acid (GA). Xylonic acid can be used in food, pharmaceutical and chemical industries as it has similar characteristics as sugar acids. Some of the applications include the function as complexing agent or chelator, in dispersal of concrete and as precursor for compounds such as co-polyamides, hydrogels and 1,2,4-butanetriol. In this study, recombinant Escherichia coli (E.coli) BL21 (DE3) was used to produce xylonic acid by manipulating a few parameters such as growth medium; Luria Bertani (LB), Super Optimal Broth (SOB),Terrific Broth (TB), M9 Minimal Medium (M9), and 2×Yeast-Tryptone (2×YT), nitrogen sources (yeast and tryptone concentration), inducer of isopropyl ß-D-1-thiogalactopyranoside (IPTG) concentration (0.01, 0.05, 0.1, 0.5 and 1.0 mM), induction time (2, 3, 4, 5 and 6 h), substrate concentration as well as comparison of substrate from difference sources. Fermentation samples were analysed using hydroxamate method and DNS method to determine XA and xylose concentration from fermentation process at 24 h, 200 rpm and 37 °C. The recombinant E.coli BL21 (DE3) was exploited as an alternative to the existing producers, with the ability to enhance the conversion of xylose to xylonic acid, fast growth, easy to handle and robust. From the study, the best culture conditions for XA production in recombinant E.coli BL21 (DE3) was achieved in Super Optimal Broth (SOB) medium, 20 g/L of tryptone, 5 g/L of yeast extract, 0.5 mM of IPTG with 2 h induction time and 10 g/L of D-xylose as substrate. Concentration of XA as high as 8.69 g/L from 10 g/L xylose was obtained when fermentation was governed under the best culture conditions when comparing with oil palm frond (OPF) hydrolysate. The result during the fermentation of OPF hydrolysate shows low concentration of XA. This is due to the limitation of xylose efficiency metabolism which affected the growth rate of recombinant E. coli BL21 (DE3). These results suggest a promising industrial-scale production of XA from recombinant E. coli BL21 (DE3). |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
College of Engineering |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Thesis |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |