MARC details
| 000 -LEADER |
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06190ntm a2200361 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251125110158.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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t||||fr|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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221215t20222022my a|||fr|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0009508(Local) |
| Qualifying information |
Hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| Language of cataloging |
eng |
| Transcribing agency |
UMP |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
KK .L56 2022 r Thesis |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Ling, Fiona Wang Ming, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Biocatalysts synthesis and deposition in microfluidics flow system / |
| Statement of responsibility, etc. |
Fiona Ling Wang Ming |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Kuantan, Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMP, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2022 |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Date of production, publication, distribution, manufacture, or copyright notice |
©2021 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvii, 165 pages : |
| Other physical details |
illustration ; |
| Dimensions |
30 cm. + |
| Accompanying material |
1 CD-ROM |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| Source |
rda |
| File type |
text file |
| Encoding format |
PDF |
| 500 ## - GENERAL NOTE |
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| General note |
College of Engineering |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Doctor of Philosophy) -- Universiti Malaysia Pahang – 2022 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The production of effective solid biocatalyst through immobilizing the enzymes on a carrier is one of the important applications in catalysis. Nanoparticles are widely used as the support for enzymes where the properties (morphology and pore size) of the solid surface and the particle's size play an important role in deciding the enzymes immobilization yield on the solid which directly affecting the catalytic performance of the produced biocatalyst. Thus, synthesizing nanoparticles with controlled size and shape (narrow particle size distribution) is an important target that large numbers of research works were aiming for. Besides that, the immobilization conditions including the time and the immobilization environment are crucial for obtaining an effective biocatalyst without causing irreversible damage to enzymes. Conventional mixing methods used in nanoparticle synthesis are one of the most effective factors in determining the quality of the nanoparticles themselves. The mass transfer area between the reactants in the conventional production methods is controlled by the mixer design and operation and that will control the reaction time which will affect the produced nanoparticle's quality. A high-precision mixing method is believed to enhance the mass transfer area and nanoparticles quality and provide an excellent platform for enzyme immobilization as well. Microfluidic technology provides a new platform in nanomaterials synthesis due to the precise handling of fluid within the microfluidic devices. Among that, the emulsion method (droplet generation) in microfluidic demonstrated various benefits including effective mixing, larger surface area, and reduction in contamination possibilities as the reactants are encapsulated in segmented droplets. The highly efficient mixing in microfluidic devices also creates a new path in immobilizing lipase on the solid carrier to produce biocatalyst. This present work aims to introduce and investigate the effect of high-precision mixing provided using microfluidics technology on silica nanoparticles synthesis and lipase immobilization for the production of nano-sized catalysts for the esterification reaction. In this work, a polydimethylsiloxane microreactor was first designed and fabricated using the direct writing method. Silica nanoparticles were synthesized in the microreactor adapting sol-gel method by varying nonionic Sorbitan Monooleate surfactant concentrations (1 – 5 vol/vol%), and residence time, and the results were compared with nanoparticles produced from a bench-scale system. The synthesized silica nanoparticles were characterized using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDX), and X-ray diffraction (XRD), and nitrogen physisorption analysis. The nanoparticles were then immobilized with Rhizomucor miehei lipase in both batch-scale and microreactor system methods. The buffer solution was collected periodically and brought for lipase activity assay analysis. The biocatalysts were characterized using Fourier-transform infrared spectroscopy (FTIR). The catalytic performance of the synthesized biocatalysts was investigated and validated through the esterification of phytosterol and quantitative analysis was conducted using gas chromatography. From the results, the silica nanoparticles produced from the microflow system were smaller in size with higher monodispersity and perfect spherical configuration compared to the irregular shapes of silica nanoparticles synthesized in bulk. The calcined silica nanoparticles produced in the bench-scale and microflow system had a mean size of 1.9 μm and 480 nm, respectively when observed using SEM. The characterization results confirmed high-quality silica nanoparticles were synthesized with smaller size, higher monodispersity, and larger pore size and volume. The experimental results showed that the lipase had a higher loading capacity on silica nanoparticles synthesized in a microflow system with an vi immobilization yield of about 90%. The present work indicates that the mixing method is not the most significant parameter in lipase immobilization compared to the properties of the carriers (morphology and size) themselves. The FTIR spectrum indicated that lipase was successfully immobilized on the silica nanoparticles via physical adsorption where the immobilized lipase showed all the bands demonstrated by silica nanoparticles and free lipase. The immobilized lipase showed a high degree of esterification confirming the high reaction rate and product yield. The immobilized lipase demonstrated a maximum degree of esterification of 97.3% under reaction conditions of 2.5 mg/ml catalyst loading; molar ratio of β-sitosterol to sunflower seed oil: 1:2; 150 rpm; 50 °C; 4 hours. This present work showed the feasibility of microreactors in synthesizing smaller and uniform nanoparticles to be applicable in different fields such as reactions and drug delivery. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
College of Engineering |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Theses |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |