Optimisation and characterisation of acid soluble collagen from japanese sea bass scales using ultrasonic assisted extraction / (Record no. 99384)

MARC details
000 -LEADER
fixed length control field 03995ntm a2200373 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field MY-KuUP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20251125110730.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
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007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field ta
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 230413t20232023my a|||fr|||| 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number THE0009455(Local)
Qualifying information Hardback
040 ## - CATALOGING SOURCE
Original cataloging agency UMP
Language of cataloging eng
Transcribing agency UMP
Description conventions rda
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) FTKKP .A78 2023 r Thesis
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Arthi Srinivasan,
Relator term author.
245 10 - TITLE STATEMENT
Title Optimisation and characterisation of acid soluble collagen from japanese sea bass scales using ultrasonic assisted extraction /
Statement of responsibility, etc. Arthi Srinivasan
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Pahang :
Name of producer, publisher, distributor, manufacturer UMP,
Date of production, publication, distribution, manufacture, or copyright notice 2023
264 #2 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice © 2023
300 ## - PHYSICAL DESCRIPTION
Extent xiv, 98 pages :
Other physical details Illustration ;
Dimensions 30 cm.+
Accompanying material 1 CD ROM
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
337 ## - MEDIA TYPE
Source rdamedia
Media type term computer
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term computer disc
347 ## - DIGITAL FILE CHARACTERISTICS
Source rda
File type text file
Encoding format PDF
500 ## - GENERAL NOTE
General note Faculty of Chemical and Process Engineering Technology
502 ## - DISSERTATION NOTE
Dissertation note Thesis (Master of Science) -- Universiti Malaysia Pahang – 2022
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references
520 3# - SUMMARY, ETC.
Summary, etc. Collagen is the most abundant protein in vertebrates and invertebrates and is the major structural protein comprising about 30% of the total protein in the human body. Due to its unique biodegradability characteristics, natural abundance, good mechanical features, low density, hydrophobic behaviour, biocompatibility, environmentally friendly, and low-cost applications, collagen has been used extensively in many applications. The objective of this research aimed to extract the collagen from fish scales using acidic, pepsin hydrolysis, and ultra-sonication assisted acid and pepsin extraction methods; To optimize the ultrasonication process and to characterize the extracted collagen. Conventional collagen extraction process has been time-consuming and produced low yields. The use of ultrasound can relatively improve the extraction efficiency. This research investigated the potential of ultrasound for the extraction of collagen from seabass scales and compared yields and purities to the conventional extraction method to find an advanced method for collagen extraction. For extraction of acid-soluble collagen using ultrasound, the acetic acid concentration, time, temperature, ultrasonication time parameters were investigated to optimise performance. ANOVA demonstrated the model is significant and has a p-value less than 0.05, with the R2 was 0.91. The collagen yield for the conventional method of acid soluble and pepsin soluble collagen extraction 12.86% and 14.23%. The ultrasound-assisted extraction shows increased yield of acid soluble and pepsin soluble collagen extraction up to 13.58% and 15.36%. The characterization of the collagen is done using Fourier transforms infrared spectroscopy (FTIR) revealed that the extracted collagens with functional groups amide A, II, III represent a fingerprint for collagen structure. Thermogravimetric analysis, (TGA) showed that the weight loss occurred in two different stages. Scanning electron microscopy (SEM) was used to examine the surface morphology for collagen molecule. UV visible spectroscopy was used to determine the purity of the collagen. Additionally, the properties of collagen like solubility, viscosity, denaturation temperature have been studied. Collagens have higher solubilisation at the acid pH ranges, and PSC is more soluble than ASC and there is a decrease in solubility with the increase in NaCl concentration. The denaturation temperature (Td) of ASC, PSC, UASC, UPSC was estimated to be 30°C and 34.56°C, 33.2°C, 37.2°C, respectively. It is concluded that fish scales can be an alternative source of collagen with high purity for application in various field.
610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Chemical and Process Engineering Technology
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Universities and colleges
General subdivision Dissertations
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Thesis
General subdivision Dissertations
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Thesis
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not lost Library of Congress Classification   In Transit Reference UMPLIB GAMBANG UMPLIB GAMBANG 13/04/2023   FTKKP .A78 2023 r Thesis T000002334 13/04/2023 1 13/04/2023 Restricted Collection
  Not lost Library of Congress Classification   In Transit Reference UMPLIB PEKAN UMPLIB PEKAN 13/04/2023   CD13333 T000002335 13/04/2023 1 13/04/2023 Restricted Collection

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