MARC details
| 000 -LEADER |
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04983ntm a2200373 i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MY-KuUP |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251125110753.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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t||||fr|||| 000 0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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230720t20232023my a|||fr|||| 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
THE0009487 (Local) |
| Qualifying information |
Hardback |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
UMP |
| Language of cataloging |
eng |
| Transcribing agency |
UMP |
| Description conventions |
rda |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
FTKKP .N35 2023 r Thesis |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Najihah Yusof, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization of propolis ultrasound-assisted extract and identification of its phytochemical and wound healing properties / |
| Statement of responsibility, etc. |
Najihah Yusof |
| 264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
Pahang : |
| Name of producer, publisher, distributor, manufacturer |
UMP, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2023 |
| 264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Date of production, publication, distribution, manufacture, or copyright notice |
© 2023 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvii, 187 pages : |
| Other physical details |
Illustration ; |
| Dimensions |
30 cm.+ |
| Accompanying material |
1 CD ROM |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
computer |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
computer disc |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| Source |
rda |
| File type |
text file |
| Encoding format |
PDF |
| 500 ## - GENERAL NOTE |
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| General note |
Faculty of Chemical and Process Engineering Technology |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Doctor of Philosophy) -- Universiti Malaysia Pahang – 2023 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical reference |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Natural products like propolis have captivated endless attention among researchers due to the presence of various beneficially active compounds such as phenolic acids and flavonoids and these compounds possess anti- inflammatory, anti- oxidative, anti- microbial, anti- carcinogenic, wound healing properties and many others. However, to obtain the phenolic compounds and active phytochemicals from propolis, the crude propolis is required to undergo an extraction process first. Generally, conventional extraction method (maceration) (MAE) has been the most commonly used extraction method to extract active compounds from propolis but unfortunately, the method requires ample time and energy as well as involving high amount of solvent usage. The information on the cytotoxicity effect of the usage and its potential wound healing effect is still lacking. This study was designed to compare the efficiency of maceration (MAE) with the non- conventional extraction method (ultrasonic- assisted extraction) (UAE) through kinetic and thermodynamic studies and to investigate the use Response Surface Methodology (RSM) to ultrasonic- assisted extraction on the extraction of total phenolic content (TPC) from Malaysian propolis from Geniotrigona thoracica stingless bees as well as its potential wound healing capability. Total phenolic and total flavonoid content analyses, α, α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) free radical scavenging assays and Ferric Reducing Ability of Plasma (FRAP) assay were implemented to determine the antioxidant activity of propolis extracts. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) analysis and scratch assays were done on EA.hy926 cells to study the cytotoxicity effect and potential wound healing activity of propolis extracts. The kinetics and thermodynamics results of ultrasonic-assisted extraction were; Ea; 0.032kJ/mol, ΔH#: -2.7796 to -2.4470 kJ/mol, ΔS#: ~8.22 J/kmol and ΔG#: -5559.16 to -4894.01 J, whereas, maceration’s Ea: 68.13 kJ/mol, ΔH#: 65.3160 to 65.6486 kJ/mol, ΔS#: -220.19 to -193.16 J/kmol and ΔG#: 130633.08 to 131298.25 J. The optimization resulted in TPC (coded), Y = 0.082 + 0.013X1 + 0.006114X2 + 0.007554X3 – 0.001739X1X2 – 0.00725X1X3 – 0.0006892X2X3 + 0.006103X12 – 0.001487X22 – 0.003858X32and the optimum conditions for ethanol concentration (X1) was 80%, extraction time (X2) was 25 minutes and extraction temperature (X3) was 60ºC which yielded 0.1007 ± 0.272 mg GAE/ g (experimental) and 0.1041 mg GAE/g (predicted) of TPC. The highest TPC was found from 80% ethanolic propolis extract; TPC: 8.8975 ± 0.008 mg GAE/g, and the highest TFC was found from 50% extract, which was 0.0276 ± 0.0335 mg QE/g. For the free radical scavenging activity of DPPH and ABTS+, the IC50 values were 0.0584 mg TE/ml and 0.3979 mg TE/ml, respectively. 20% ethanolic propolis extract was non-toxic to cells while 50% and 80% extracts were toxic with IC50 of 97.5 μg/ml and 58 μg/ml individually. In scratch assay, the EA.hy926 cells were treated with 20% of ethanolic propolis extracts and formed monolayers after 18 hours of incubation, whereas, the untreated wound (negative control) was completely closed after 24 hours. This study revealed that UAE was more efficient and economical than MAE in the TPC extraction from propolis, there was a synergistic effect of ethanol concentration, extraction time and extraction temperature has generated the highest TPC, propolis exhibited antioxidant activity, cytotoxicity and potential wound healing capability at a certain concentration on EA.hy926cells. |
| 610 20 - SUBJECT ADDED ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Faculty of Chemical and Process Engineering Technology |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Universities and colleges |
| General subdivision |
Dissertations |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Thesis |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |