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Molecular identification of a fungi isolate from liquid culture of colon cancer biopsy / Nur Shahira Binti Razali

By:

Nur Shahira Binti Razali [author.]

Material type: Text

text

Media type:

unmediated

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volume

ISBN:

THE0010315 (Local)

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Dissertation note: Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 Abstract: Fungal infections are increasingly recognized as significant factors affecting various health issues in humans, including cancer. This research aimed to explore fungal species obtained from liquid cultures derived from colon cancer biopsy samples, particularly seeking to identify possible connections between fungal presence and colon cancer pathology. Liquid cultures from biopsies were carefully prepared, and fungal isolates were grown under regulated laboratory conditions. Morphological traits and growth behaviors were documented to ensure precise differentiation of the isolates. Molecular classification of the fungi was conducted utilizing Polymerase Chain Reaction (PCR)- based methods that target specific genetic markers of fungi. DNA sequencing and phylogenetic analysis additionally validated the taxonomic identification of the isolates. The findings indicated the presence of Gymnopilus sp. within the biopsy cultures, with some isolates exhibiting distinct genetic variations. Initial assessments imply possible interactions between the identified fungi and the tumor microenvironment, warranting further study. This research emphasizes the significance of investigating fungal microbiota in cancer studies and highlights the critical need for molecular identification techniques to uncover potential microbial contributors to cancer development. Future research will aim to clarify the mechanistic roles of identified fungal species in the progression of colon cancer, providing insights into innovative diagnostic and therapeutic strategies.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Restricted Collection	UMPLIB GAMBANG	FIST .H57 2025 r Bc. (Browse shelf(Opens below))	Not for loan		T000003694

Faculty of Industrial Sciences and Technology

Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025

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Fungal infections are increasingly recognized as significant factors affecting various health issues in humans, including cancer. This research aimed to explore fungal species obtained from liquid cultures derived from colon cancer biopsy samples, particularly seeking to identify possible connections between fungal presence and colon cancer pathology. Liquid cultures from biopsies were carefully prepared, and fungal isolates were grown under regulated laboratory conditions. Morphological traits and growth behaviors were documented to ensure precise differentiation of the isolates. Molecular classification of the fungi was conducted utilizing Polymerase Chain Reaction (PCR)- based methods that target specific genetic markers of fungi. DNA sequencing and phylogenetic analysis additionally validated the taxonomic identification of the isolates. The findings indicated the presence of Gymnopilus sp. within the biopsy cultures, with some isolates exhibiting distinct genetic variations. Initial assessments imply possible interactions between the identified fungi and the tumor microenvironment, warranting further study. This research emphasizes the significance of investigating fungal microbiota in cancer studies and highlights the critical need for molecular identification techniques to uncover potential microbial contributors to cancer development. Future research will aim to clarify the mechanistic roles of identified fungal species in the progression of colon cancer, providing insights into innovative diagnostic and therapeutic strategies.